We studied the effect of nitinol, the most prevalent material for endovascular stents, on metabolic and coagulation activity of a primary culture of human umbilical vein endothelial cells (HUVEC). Metabolic activity was evaluated using MTS-test and by the level of stable NO metabolites in the conditioned medium, coagulation activity was assessed by activity of von Willebrand factor (vWF) and levels of plasminogen activator inhibitor-1 (PAI-1) and soluble endothelial protein C receptors (sEPCR). Exposure to nitinol reduced metabolic activity of the cell culture by 11.1% in comparison with the control (p<0.001). Although absolute activity of vWF and absolute level of sEPCR were elevated, incubation with nitinol did not lead to a statistically significant elevation of these parameters in comparison with the control, which can indicate the absence of substantial hypercoagulation effects of nitinol.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 496-499, April, 2021
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Kalinin, R.E., Suchkov, I.A., Mzhavanadze, N.D. et al. Effect of Nitinol on Metabolic and Coagulation Activity of Endothelial Cells Culture. Bull Exp Biol Med 171, 480–482 (2021). https://doi.org/10.1007/s10517-021-05255-8
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DOI: https://doi.org/10.1007/s10517-021-05255-8