The quinoid pigment echinochrome A isolated from the sea urchin Scaphechinus mirabilis, the product of its oxidation dehydroechinochrome, and structurally similar antiviral agent oxolin were tested for their ability to inhibit plaque formation induced by herpes simplex virus type 1 (HSV-1) in Vero cells. The tested compounds showed significant anti-HSV-1 activity, mainly due to their direct effect on viral particles and on virus attachment to cells. The antiviral efficacy of the test compounds increased in the following order: oxolin→echinochrome A→dehydroechinochrome.
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References
Levina VI, Burtsev AS, Arzamastsev AP, Grigoriev NB, Granik VG. Polarographic study of redox and hydrolytic transformations of the virucidal drug “Oxolin”. Vopr. Biol. Med. Farm. Khimii. 2003;(1):40-45. Russian.
Novikov VL, Shestak OP, Mishchenko NP, Fedoreev SA, Vasileva EA, Glazunov VP, Artyukov AA. Oxidation of 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone (echinochrome a) by atmospheric oxygen 1. Structure of dehydroechinochrome. Russ. Chem. Bull. 2018;67(2):282-290.
Handbook of Virology: Viruses and Viral Infections in Humans and Animals. L’vov DK, ed. Moscow, 2013. Russian.
Berridge MV, Herst PM, Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol. Annu. Rev. 2005;11:127-152. doi: https://doi.org/10.1016/S1387-2656(05)11004-7
Fedoreyev SA, Krylova NV, Mishchenko NP, Vasileva EA, Pislyagin EA, Iunikhina OV, Lavrov VF, Svitich OA, Ebralidze LK, Leonova GN. Antiviral and antioxidant properties of echinochrome A. Mar. Drugs. 2018;16(12):509. doi: https://doi.org/10.3390/md16120509.
James C, Harfouche M, Welton NJ, Turner KME, Abu-Raddad LJ, Gottlieb SL, Looker KJ. Herpes simplex virus: global infection prevalence and incidence estimates, 2016. Bull. WHO. 2020;98:315-329. doi: http://dx.doi.org/https://doi.org/10.2471/BLT.19.237149
Marcocci ME, Amatore D, Villa S, Casciaro B, Aimola P, Franci G, Grieco P, Galdiero M, Palamara A.T, Mangoni ML, Nencioni L. The amphibian antimicrobial peptide temporin B inhibits in vitro Herpes Simplex Virus 1 infection. Antimicrob. Agents Chemother. 2018;62(5):e02367-17. doi: https://doi.org/10.1128/AAC.02367-17
Mischenko NP, Fedoreyev SA, Pokhilo ND, Anufriev VP, Denisenko VA, Glazunov VP. Echinamines A and B, first aminated hydroxynaphthazarins from the sea urchin Scaphechinus mirabilis. J. Nat. Prod. 2005;68(9):1390-1393. doi: https://doi.org/10.1021/np049585r
Mishchenko NP, Krylova NV, Iunikhina OV, Vasileva EA, Likhatskaya GN, Pislyagin EA, Tarbeeva DV, Dmitrenok PS, Fedoreyev SA. Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1. Mar. Drugs. 2020;18(11):550. doi: https://doi.org/10.3390/md18110550
Mishchenko NP, Vasileva EA, Gerasimenko AV, Grigorchuk VP, Dmitrenok PS, Fedoreyev SA. Isolation and structure determination of echinochrome A oxidative degradation products. Molecules. 2020;25(20):4778. doi: https://doi.org/10.3390/molecules25204778.
Tai CJ, Li CL, Tai CJ, Wang CK, Lin LT. Early Viral Entry Assays for the identification and evaluation of antiviral compounds. J. Vis. Exp. 2015;(105):e53124. doi: https://doi.org/10.3791/53124
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 477-480, April, 2021
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Iunikhina, O.V., Krylova, N.V., Mishchenko, N.P. et al. Comparative In Vitro Study of Antiherpetic Activity of Echinochrome A and Product of Its Oxidation Dehydroechinochrome. Bull Exp Biol Med 171, 464–467 (2021). https://doi.org/10.1007/s10517-021-05251-y
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DOI: https://doi.org/10.1007/s10517-021-05251-y