To better explore the pathophysiology of FA and its therapy, we aimed to establish a simple and practicable FA model with Freund’s adjuvant and introduce an easy and reliable laboratory evaluation method for assessment of inflammation in intestinal segments at different anatomical locations. BALB/c mice were sensitized with ovalbumin combined with Freund’s adjuvant. Complete Freund’s adjuvant was chosen for the first sensitization and two weeks later incomplete Freund’s adjuvant was used for a second sensitization. Two weeks later, the sensitized mice were challenged with 50 mg ovalbumin every other day. After the 6 challenge, all mice were assessed for systemic anaphylaxis, and then sacrificed for sample collection. All sensitized mice showed anaphylactic symptoms and markedly increased levels of serum ovalbumin-specific IgE and IgG1. The activity of mast cell protease-1 (mMCPT-1) was significantly increased in the serum and interstitial fluid of the duodenum, jejunum, ileum, and colon. A successful FA model was established, of which inflammation occurred in the duodenum, jejunum, ileum, and colon. This model provides a reliable and simple tool for analysis of the mechanism of FA and methods of immunotherapy. Moreover, combined detection of ovalbumin-specific antibody and local mMCPT-1 levels could potentially be used as the major indicator for assessment of food allergy.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 3, pp. 333-338, March, 2021
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Zhang, Y., Liu, J.Y., Shao, J.W. et al. Effective Model of Food Allergy in Mice Sensitized with Ovalbumin and Freud’s Adjuvant. Bull Exp Biol Med 171, 352–356 (2021). https://doi.org/10.1007/s10517-021-05226-z
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DOI: https://doi.org/10.1007/s10517-021-05226-z