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Influence of Sucrose on the Efficiency of Cryopreservation of Human Umbilical Cord-Derived Multipotent Stromal Cells with the Use of Various Penetrating Cryoprotectants

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the influence of sucrose applied in combination with different concentrations of penetrating cryoprotectants (DMSO, ethylene glycol, and glycerol) on the efficiency of cryopreservation of umbilical cord-derived multipotent stromal cells (MSC). The results indicate that these cells can be cryopreserved with the use of 5-10% DMSO or ethylene glycol with equal efficiency; addition of 0.2 M sucrose does not affect cell survival after thawing. The efficiency of glycerol as a cryoprotectant increases with increasing its concentration from 5 to 10%, but remains significantly lower than the efficiency of DMSO or ethylene glycol. Addition of sucrose to a final concentration of 0.2 M increases the efficiency of glycerol. The efficiency of combination of 10% glycerol and sucrose was comparable with that of combinations of DMSO and ethylene glycol with sucrose. The mechanism of the observed enhancement is apparently related to the influence of sucrose on the dynamic properties of the lipid membranes and facilitation of glycerol diffusion into the cells.

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Authors and Affiliations

  1. V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    I. V. Arutyunyan & A. V. Elchaninov

  2. Research Institute of Human Morphology, Moscow, Russia

    E. Yu. Kananykhina & T. Kh. Fatkhudinov

  3. N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    A. V. Elchaninov

  4. The Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia

    T. Kh. Fatkhudinov

Authors
  1. I. V. Arutyunyan
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  2. E. Yu. Kananykhina
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  3. A. V. Elchaninov
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  4. T. Kh. Fatkhudinov
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Corresponding author

Correspondence to T. Kh. Fatkhudinov.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 47-53, March, 2021

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Arutyunyan, I.V., Kananykhina, E.Y., Elchaninov, A.V. et al. Influence of Sucrose on the Efficiency of Cryopreservation of Human Umbilical Cord-Derived Multipotent Stromal Cells with the Use of Various Penetrating Cryoprotectants. Bull Exp Biol Med 171, 150–155 (2021). https://doi.org/10.1007/s10517-021-05187-3

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  • DOI: https://doi.org/10.1007/s10517-021-05187-3

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