Skip to main content
Log in

Radiation-Chemical Biotechnology for Producing Ultrashort (50-100n) Single-Chain Polydeoxyribonucleotides with Anticancer Activity

  • BIOTECHNOLOGIES
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The fundamental possibility of obtaining ultrashort (50n-100n) single-stranded polydeoxyribonucleotides by the method of radiation-chemical destruction of total yeast DNA was established and their anticancer activity was shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Buchvostov AA, Orlov AP, Shatalov OA, Kusnetsov DA. Unique beta-like DNA polymerase from chromatin of human acute myeloid leukemia HL-60 cells. Geny Kletki. 2014;9(2):46-52. Russian.

  2. Kabanov VYa, Ershov BG, Feldman VI, Polikarpov AI, Kiryukhin DP, Apel’ PYu. Radiation chemistry of polymers. High Energy Chemistry. 2009;43(1):1-18.

  3. Matniyazov RT, Chemeris DA, Kuluev AR, Zubov VV, Chemeris AV. Diversity of the methods for obtaining random fragmented DNA. Biomika. 2014;6(3):155-166. Russian.

  4. Polidanov MA, Blokhin IS, Skorokhod AA, Alieva SG, Typikin DV, Shcherbakova IV. Method of agarose gel electrophoresis. Modern Science. 2020;(3-2):69-72. Russian.

  5. Kuznetsov AA. Biophysics of Ionizing and Non-Ionizing Radiation. Vladimir, 2016. Russian.

  6. Treshchalina EM, Zhukova OS, Gerasimova GK, Andronova NV, Garin AM. Methodological guidelines for the study of the antitumor activity of pharmacological substances. Manual on Experimental (Preclinical) Study of New Pharmacological Substances, Khabriev RU, ed. Moscow, 2005. P. 637-651. Russian.

  7. Bukhvostov AA, Shatalov OA, Orlov AP, Kuznetsov DA. An atypical DNA polymerase beta overexpressed in human AML/HL-60 malignant cells. J. Cancer Sci. Ther. 2013;5(2):094-099. https://doi.org/10.4172/1948-5956.1000191

    Article  CAS  Google Scholar 

  8. Brezis H, Pazy A. Convergence and approximation of semigroups of nonlinear operators in Banach spaces. J. Func. Anal. 1972;9(1):63-74. https://doi.org/10.1016/0022-1236(72)90014-6

    Article  Google Scholar 

  9. Ermakov KV, Bukhvostov AA, Vedenkin AS, Stovbun SV, Dvornikov AS, Kuznetsov DA. Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report. Acta Medica (Hradec Kralove). 2019;62(4):170-173. https://doi.org/10.14712/18059694.2020.8

  10. Head SR, Komori HK, LaMere SA, Whisenant T, Van Nieuwerburgh F, Salomon DR, Ordoukhanian P. Library construction for next-generation sequencing: overviews and challenges. Biotechniques. 2014;56(2):61-64, 66, 68, passim. https://doi.org/10.2144/000114133

  11. Nasr P, Delorme T. Age-Dependent vulnerability of the striatal mitochondrial to 3-nitropropionic acid. Ohio J. Sci. 2007;107(5):120-124.

    CAS  Google Scholar 

  12. Olins AL, Herrmann H, Lichter P, Olins DE. Retinoic acid differentiation of HL-60 cells promotes cytoskeletal polarization. Exp. Cell Res. 2000;254(1):130-142. https://doi.org/10.1006/excr.1999.4727

    Article  CAS  PubMed  Google Scholar 

  13. Roy MK, Thalang VN, Trakoontivakorn G, Nakahara K. Mechanism of mahanine-induced apoptosis in human leukemia cells (HL-60). Biochem. Pharmacol. 2004;67(1):41-51. https://doi.org/10.1016/j.bcp.2003.07.021

    Article  CAS  PubMed  Google Scholar 

  14. Stovbun SV, Ermakov KV, Bukhvostov AA, Vedenkin AS, Kuznetsov DA. Primer-Like Inhibitors for DNA Repair Enzymes of the AML-HL60 and WERI-1A/Y79 Malignant Cells. Adv. Enzyme Res. 2019;7(3):27-44. https://doi.org/10.4236/aer.2019.73003

    Article  CAS  Google Scholar 

  15. Stovbun S, Vedenkin A, Kuznetsov D, Ermakov K, Bukhvostov A. A new DNA repair-related platform for pharmaceutical outlook in cancer therapies: ultrashort single-stranded polynucleotides. Scientia Pharmaceutica. 2019;87(4):25-27. https://doi.org/10.3390/scipharm87040025

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. G. Mikhaleva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 1, pp. 92-94, January, 2021

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Stovbun, S.V., Vedenkin, A.S., Mikhaleva, M.G. et al. Radiation-Chemical Biotechnology for Producing Ultrashort (50-100n) Single-Chain Polydeoxyribonucleotides with Anticancer Activity. Bull Exp Biol Med 171, 74–76 (2021). https://doi.org/10.1007/s10517-021-05175-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-021-05175-7

Key Words

Navigation