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Noninvasive Laser Microtomography of a Human Isolated Oocyte

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Using a non-invasive approach, quantitative laser scanning microtomography (QLSM), the morphology of human oocyte was studied layer-by-layer. Then, the cell volume was computed based on 3D reconstruction of a stack of optical sections obtained by QLSM. The integrity of oocyte membrane after cryopreservation was assessed by measuring the changes in oocyte volume in response to hypotonic shock.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia

    A. G. Pogorelov & V. N. Pogorelova

  2. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    N. P. Makarova, A. P. Sysoeva & G. T. Sukhikh

Authors
  1. A. G. Pogorelov
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  2. N. P. Makarova
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  3. A. P. Sysoeva
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  4. V. N. Pogorelova
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  5. G. T. Sukhikh
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Corresponding author

Correspondence to A. G. Pogorelov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 1, pp. 43-48, January, 2021

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Pogorelov, A.G., Makarova, N.P., Sysoeva, A.P. et al. Noninvasive Laser Microtomography of a Human Isolated Oocyte. Bull Exp Biol Med 171, 32–36 (2021). https://doi.org/10.1007/s10517-021-05166-8

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  • DOI: https://doi.org/10.1007/s10517-021-05166-8

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