Skip to main content
SpringerLink
Log in

Study of Translocation of Stabilized NO Forms through Rat Skin using Electron Paramagnetic Resonance Method

  • BIOPHYSICS AND BIOCHEMISTRY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

We studied the effect of dinitrosyl-iron complexes with N-acetyl-L-cysteine as a thiol-containing ligand (DNIC-Acc) after transdermal administration to rats. Electron paramagnetic resonance spectroscopy with a lipophilic NO spin trap (a complex of iron and diethyldithiocarbamate ions) showed that DNIC-Acc administration significantly increased the total level of NO in the lung and liver tissues of the animal, which was accompanied by a slight decrease in the mean BP (<10%).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Timoshin AA, Drobotova DY, Tskitishvili OV, Serebryakova LI, Pisarenko OI, Ruuge EK, Vanin AF. Protective effect of dinitrosyl-iron complexes with glutathione in rat myocardial regional ischemia: a microdialysis assay study. Dokl. Biochem. Biophysics. 2010;432(1):106-109.

    Article  CAS  Google Scholar 

  2. Timoshin AA, Lakomkin VL, Abramov AA, Ruuge EK, Vanin AF. The biological effect of dinitrosyl iron complexes with glutathione upon nitric oxide hyperproduction induced by endotoxin shock. Biophysics. 2019;64(1):89-94.

    Article  CAS  Google Scholar 

  3. Timoshin AA, Lakomkin VL, Abramov AA, Ruuge EK, Vanin AF. Hypotensive Effect and Accumulation of Dinitrosyl Iron Complexes in Blood and Tissues after Intravenous and Subcutaneous Injection. Bull. Exp. Biol. Med. 2016;162(2):207-210. doi: https://doi.org/10.1007/s10517-016-3577-x.

    Article  CAS  PubMed  Google Scholar 

  4. Chen YJ, Ku WC, Feng LT, Tsai ML, Hsieh CH, Hsu WH, Liaw WF, Hung CH, Chen YJ. Nitric Oxide Physiological Responses and Delivery Mechanisms Probed by Water-Soluble Roussin’s Red Ester and {Fe(NO)2}10 DNIC. J. Am. Chem. Soc. 2008;130(33):10 929-10 938. doi: https://doi.org/10.1021/ja711494m

    Article  CAS  Google Scholar 

  5. Ke CH, Chen CH, Tsai ML, Wang HC, Tsai FT, Chiang YW, Shih WC, Bohle DS, Liaw WF. {Fe(NO)(2)(9) Dinitrosyl Iron Complex Acting as a Vehicle for the NO Radical. J. Am. Chem. Soc. 2017;139(1):67-70. doi: https://doi.org/10.1021/jacs.6b11454

  6. Rahmanto YS, Kalinowski DS, Lane DJR, Lok HC, Richardson V, Richardson DR. Nitrogen Monoxide (NO) Storage and Transport by Dinitrosyl-Dithiol-Iron Complexes: Long-Lived NO That Is Trafficked by Interacting Proteins. J. Biol. Chem. 2012;287(10):6960-6968. doi: https://doi.org/10.1074/jbc.R111.329847

    Article  CAS  Google Scholar 

  7. Sen CK. Nutritional biochemistry of cellular glutathione. J. Nutr. Biochem. 1997;8(12):660-672.

    Article  CAS  Google Scholar 

  8. Shumaev KB, Dudylina AL, Ivanova MV, Pugachenko IS, Ruuge EK. Dinitrosyl Iron Complexes: Formation and Antiradical Action in Heart Mitochondria. Biofactors. 2018;44(3):237-244. doi: https://doi.org/10.1002/biof.1418

    Article  CAS  PubMed  Google Scholar 

  9. Shumaev KB, Gubkin AA, Serezhenkov VA, Lobysheva II, Kosmachevskaya OV, Ruuge EK, Lankin VZ, Topunov AF, Vanin AF. Interaction of Reactive Oxygen and Nitrogen Species With Albumin- And Methemoglobin-Bound Dinitrosyl-Iron Complexes. Nitric Oxide. 2008;18(1):37-46. doi: https://doi.org/10.1016/j.niox.2007.09.085

    Article  CAS  PubMed  Google Scholar 

  10. Støttrup NB, Løfgren B, Birkler RD, Nielsen JM, Wang L, Caldarone CA, Kristiansen SB, Contractor H, Johannsen M, Bøtker HE, Nielsen TT. Inhibition of the Malate-Aspartate Shuttle by Pre-Ischaemic Aminooxyacetate Loading of the Heart Induces Cardioprotection. Cardiovasc. Res. 2010;88(2): 257-266. doi: https://doi.org/10.1093/cvr/cvq205

    Article  CAS  PubMed  Google Scholar 

  11. Timoshin AA, Vanin AF, Orlova TR, Sanina NA, Ruuge EK, Aldoshin SM, Chazov EI. Protein-bound Dinitrosyl-Iron Complexes Appearing in Blood of Rabbit Added With a Low-Molecular Dinitrosyl-Iron Complex: EPR Studies. Nitric Oxide. 2007;16(2):286-293. doi: https://doi.org/10.1016/j.niox.2006.09.005

    Article  CAS  PubMed  Google Scholar 

  12. Timoshin AА, Lakomkin VL, Аbramov AА, Ruuge EK, Kapel’ko VI, Chazov EI, Vanin AF. The Hypotensive Effect of the Nitric Monoxide Donor Oxacom at Different Routs of Its Administration to Experimental Animals. Eur. J. Pharmacol. 2015;765:525-532. doi: https://doi.org/10.1016/j.ejphar.2015.09.011

    Article  CAS  Google Scholar 

  13. Vanin AF, Poltorakov AP, Mikoyan VD, Kubrina LN, Burbaev DS. Polynuclear Water-Soluble Dinitrosyl Iron Complexes With Cysteine or Glutathione Ligands: Electron Paramagnetic Resonance and Optical Studies. Nitric Oxide. 2010;23(2):136-149. doi: https://doi.org/10.1016/j.niox.2010.05.285

    Article  CAS  PubMed  Google Scholar 

  14. Vanin AF, Timoshin AA. Determination of in Vivo Nitric Oxide Levels in Animal Tissues Using a Novel Spin Trapping Technology. Methods Mol. Biol. 2011;704:135-149. doi: https://doi.org/10.1007/978-1-61737-964-2_11

    Article  CAS  PubMed  Google Scholar 

  15. Wu SC, Lu CY, Chen YL, Lo FC, Wang TY, Chen YJ, Yuan SS, Liaw WF, Wang YM. Water-Soluble Dinitrosyl Iron Complex (DNIC): A Nitric Oxide Vehicle Triggering Cancer Cell Death via Apoptosis. Inorg Chem. 2016;55(18):9383-9392. doi: https://doi.org/10.1021/acs.inorgchem.6b01562

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Medical Research Centre for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia

    A. A. Timoshin, K. B. Shumaev, V. L. Lakomkin, A. A. Abramov & E. K. Ruuge

  2. A. N. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russia

    K. B. Shumaev

  3. Faculty of Physics, M. V. Lomonosov Moscow State University, Moscow, Russia

    E. K. Ruuge

Authors
  1. A. A. Timoshin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. B. Shumaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. L. Lakomkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Abramov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. K. Ruuge
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. A. Timoshin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 9, pp. 290-295, September, 2020

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Timoshin, A.A., Shumaev, K.B., Lakomkin, V.L. et al. Study of Translocation of Stabilized NO Forms through Rat Skin using Electron Paramagnetic Resonance Method. Bull Exp Biol Med 170, 303–307 (2021). https://doi.org/10.1007/s10517-021-05056-z

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-021-05056-z

Key Words

Search

Navigation