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In Vitro Effects of Lithium Carbonate on Cell Cycle, Apoptosis, and Autophagy in Hepatocellular Carcinoma-29 Cells

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We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro. Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration of 5 mM lithium carbonate. Induction of autophagy in HCC-29 cells was detected by transmission electron microscopy and immunofluorescence staining. Thus, lithium carbonate produces an antitumor effect by arresting cell cycle in the G2/M-phase and induction of apoptosis and autophagy in HCC-29 cells, which confirms the lithium potential as a promising drug for the treatment of hepatocellular carcinoma.

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References

  1. Bgatova NP, Gavrilova YuS, Lykov AP, Solovieva AO, Makarova VV, Borodin YuI, Konenkov VI. Apoptosis and autophagy in hepatocarcinoma cells induced by different forms of lithium salts. Tsitologiya. 2017;59(3):178-184. Russian.

    CAS  Google Scholar 

  2. Gavrilova YuS, Bgatova NP, Solovieva AO, Trifonova KE, Lykov AP, Borodin YuI, Konenkov VI. The target cells of different lithium forms in heterogeneous population hepatocarcinoma-29. Tsitologiya. 2016;58(3):186-191. Russian.

    Google Scholar 

  3. Kaledin VI, Zhukova NA, Nikolin VP, Popova NA, Beliaev MD, Baginskaya NV, Litvinova EA, Tolstikova TG, Lushnikova EL, Semenov DE. Hepatocarcinoma-29, a metastasizing transplantable mouse tumor inducing cachexia. Bull. Exp. Biol. Med. 2009;148(6):903-908. https://doi.org/10.1007/s10517-010-0848-9

    Article  CAS  PubMed  Google Scholar 

  4. Taskaeva IS, Bgatova NP. Lithium salts in experimental oncology (review). Sib. Nauch. Med. Zh. 2019;39(5):12-18. Russian.

    Google Scholar 

  5. Dash S, Chava S, Chandra PK, Aydin Y, Balart LA, Wu T. Autophagy in hepatocellular carcinomas: from pathophysiology to therapeutic response. Hepat. Med. 2016;8:9-20. https://doi.org/10.2147/HMER.S63700

    Article  PubMed  PubMed Central  Google Scholar 

  6. Degterev A, Yuan J. Expansion and Evolution of Cell Death Programmes. Nat. Rev. Mol. Cell. Biol. 2008;9(5):378-390. https://doi.org/10.1038/nrm2393

    Article  CAS  PubMed  Google Scholar 

  7. Gao S, Li X, Ding X, Qi W, Yang Q. Cepharanthine Induces Autophagy, Apoptosis and Cell Cycle Arrest in Breast Cancer Cells. Cell. Physiol. Biochem. 2017;41(4):1633-1648. https://doi.org/10.1159/000471234

    Article  CAS  PubMed  Google Scholar 

  8. Jain MV, Paczulla AM, Klonisch T, Dimgba FN, Rao SB, Roberg K, Schweizer F, Lengerke C, Davoodpour P, Palicharla VR, Maddika S, Łos M. Interconnections Between Apoptotic, Autophagic and Necrotic Pathways: Implications for Cancer Therapy Development. J. Cell. Mol. Med. 2013;17(1):12-29. https://doi.org/10.1111/jcmm.12001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Lin W, Xie J, Xu N, Huang L, Xu A, Li H, Li C, Gao Y, Watanabe M, Liu C, Huang P. Glaucocalyxin A Induces G2/M Cell Cycle Arrest and Apoptosis Through the PI3K/Akt Pathway in Human Bladder Cancer Cells. Int. J. Biol. Sci. 2018;14(4):418-426. https://doi.org/10.7150/ijbs.23602

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Mathiassen SG, De Zio D, Cecconi F. Autophagy and the Cell Cycle: A Complex Landscape. Front. Oncol. 2017;7:51. https://doi.org/10.3389/fonc.2017.00051

  11. Ojha R, Ishaq M, Singh SK. Caspase-mediated crosstalk between autophagy and apoptosis: Mutual adjustment or matter of dominance. J. Cancer Res. Ther. 2015;11(3):514-524. https://doi.org/10.4103/0973-1482.163695

    Article  CAS  PubMed  Google Scholar 

  12. Quiroz JA, Gould TD, Manji HK. Molecular effects of lithium. Mol. Interv. 2004;4(5):259-272. https://doi.org/10.1124/mi.4.5.6

    Article  CAS  PubMed  Google Scholar 

  13. Sarkar S, Floto RA, Berger Z, Imarisio S, Cordenier A, Pasco M, Cook LJ, Rubinsztein DC. Lithium induces autophagy by inhibiting inositol monophosphatase. J. Cell. Biol. 2005;170(7):1101-1011. https://doi.org/10.1083/jcb.200504035

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology — Affiliated Branch of Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    Y. S. Taskaeva, N. P. Bgatova, A. O. Solovieva & S. M. Miroshnichenko

  2. Novosibirsk National Research State University, Novosibirsk, Russia

    Y. S. Taskaeva

  3. Abai Kazakh National Pedagogical University, Almaty, Republic of Kazakhstan

    R. S. Dossymbekova & Z. B. Tungushbaeva

  4. Research Institute of Biochemistry — Structural Subdivision of Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia

    S. M. Miroshnichenko

  5. S. D. Asfendiyarov Kazakh National Medical University, Almaty, Republic of Kazakhstan

    K. O. Sharipov

Authors
  1. Y. S. Taskaeva
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  2. N. P. Bgatova
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  3. R. S. Dossymbekova
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  4. A. O. Solovieva
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  5. S. M. Miroshnichenko
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  6. K. O. Sharipov
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  7. Z. B. Tungushbaeva
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Corresponding author

Correspondence to Y. S. Taskaeva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 8, pp. 221-226, August, 2020

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Taskaeva, Y.S., Bgatova, N.P., Dossymbekova, R.S. et al. In Vitro Effects of Lithium Carbonate on Cell Cycle, Apoptosis, and Autophagy in Hepatocellular Carcinoma-29 Cells. Bull Exp Biol Med 170, 246–250 (2020). https://doi.org/10.1007/s10517-020-05044-9

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  • DOI: https://doi.org/10.1007/s10517-020-05044-9

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