Skip to main content

Advertisement

SpringerLink
Log in

Mycobacterium vaccae Lysate Induces Anti-Allergic Immune Response In Vitro

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Mycobacterium vaccae is a soil saprophyte which exerts anti-allergic properties. There are data that mechanism of action of M. vaccae when used in the treatment of human and animal allergic diseases is associated with Th1-phenotype switch. Here we studied the properties of sonicated M. vaccae lysate in co-cultures of dendritic cells and CD4+T cells. M. vaccae lysate stimulated IL-10 synthesis in co-cultures and CD86 expression in dendritic cells, being more potent than heat-killed M. vaccae. The reported clinical data and the mechanism of action of M. vaccae lysate suggest that its use is a feasible option for the primary prevention of allergic diseases, in particular atopic dermatitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arkwright PD, David TJ. Effect of Mycobacterium vaccae on atopic dermatitis in children of different ages. Br. J. Dermatol. 2003;149(5):1029-1034.

    Article  CAS  Google Scholar 

  2. Bazzi S, El-Darzi E, McDowell T, Modjtahedi H, Mudan S, Achkar M, Akle C, Kadara H, Bahr GM. Defining genome-wide expression and phenotypic contextual cues in macrophages generated by granulocyte/macrophage colony-stimulating factor, macrophage colony-stimulating factor, and heat-killed mycobacteria. Front. Immunol. 2017;8:1253. https://doi.org/10.3389/fimmu.2017.01253

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Bazzi S, Modjtahedi H, Mudan S, Achkar M, Akle C, Bahr GM. Immunomodulatory effects of heat-killed Mycobacterium obuense on human blood dendritic cells. Innate Immun. 2017;23(7):592-605.

    Article  CAS  Google Scholar 

  4. Berth-Jones J, Arkwright PD, Marasovic D, Savani N, Aldridge CR, Leech SN, Morgan C, Clark SM, Ogilvie S, Chopra S, Harper JI, Smith CH, Rook GA, Friedmann PS. Killed Mycobacterium vaccae suspension in children with moderate-to-severe atopic dermatitis: a randomized, double-blind, placebo-controlled trial. Clin. Exp. Allergy. 2006;36(9):1115-1121.

    Article  CAS  Google Scholar 

  5. Davies MG, Symons C, Shaw S, Kaminski ER. An open study to assess the efficacy clinically and immunologically of M. vaccae vaccine in patients with atopic dermatitis. J. Dermatolog. Treat. 2006;17(2):74-77.

    Article  CAS  Google Scholar 

  6. Dunstan JA, Brothers S, Bauer J, Hodder M, Jaksic MM, Asher MI, Prescott SL. The effects of Mycobacteria vaccae derivative on allergen-specific responses in children with atopic dermatitis. Clin. Exp. Immunol. 2011;164(3):321-329.

    Article  CAS  Google Scholar 

  7. EMA/502527/2019. Bacterial lysates-containing medicinal products indicated for respiratory conditions. URL: https://www.ema.europa.eu/en/documents/referral/bacterial-lysate-medicines-article-31-referral-chmp-assessment-report_en.pdf

  8. Grönroos M, Parajuli A, Laitinen OH. Short-term direct contact with soil and plant materials leads to an immediate increase in diversity of skin microbiota. MicrobiologyOpen. 2019;8(3):e00645. https://doi.org/10.1002/mbo3.645

  9. Hassell JE Jr, Fox JH, Arnold MR, Siebler PH, Lieb MW, Schmidt D, Spratt EJ, Smith TM, Nguyen KT, Gates CA, Holmes KS, Schnabel KS, Loupy KM, Erber M, Lowry CA. Treatment with a heat-killed preparation of Mycobacterium vaccae after fear conditioning enhances fear extinction in the fear-potentiated startle paradigm. Brain Behav. Immun. 2019;81:151-160.

    Article  Google Scholar 

  10. Le Bert N, Chain BM, Rook G, Noursadeghi M. DC Priming by M. vaccae inhibits Th2 responses in contrast to specific TLR2 priming and is associated with selective activation of the CREB pathway. PLoS One. 2011;6(4):e18346. https://doi.org/10.1371/journal.pone.0018346

  11. Myles IA, Earland NJ, Anderson ED, Moore IN, Kieh MD, Williams KW, Saleem A, Fontecilla NM, Welch PA, Darnell DA, Barnhart LA, Sun AA, Uzel G, Datta SK. First-in-human topical microbiome transplantation with Roseomonas mucosa for atopic dermatitis. JCI Insight. 2018;3(9):120608. https://doi.org/10.1172/jci.insight.120608

    Article  PubMed  Google Scholar 

  12. Nesmiyanov P, Gutov M, Strygin A, Tolkachev B, Morkovin E, Dotsenko A. M. vaccae-based formulation for the primary prevention of atopic dermatitis. Allergy 2018;73, S105:107.

  13. Schülke S. Induction of Interleukin-10 producing dendritic cells as a tool to suppress allergen-specific T helper 2 responses. Front. Immunol. 2018;9:455. https://doi.org/10.3389/fimmu.2018.00455

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Súkeníková L, Černý V, Novotná O, Petrásková P, Boráková K, Kolářová L, Prokešová L, Hrdý J. Different capacity of in vitro generated myeloid dendritic cells of newborns of healthy and allergic mothers to respond to probiotic strain E. coli O83:K24:H31. Immunol. Lett. 2017;189:82-89.

  15. Zuany-Amorim C, Sawicka E, Manlius C, Le Moine A, Brunet L.R, Kemeny DM, Bowen G, Rook G, Walker C. Suppression of airway eosinophilia by killed Mycobacterium vaccae-induced allergen-specific regulatory T-cells. Nat. Med. 2002;8(6):625-629.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Volgograd State Medical University, Ministry of Health of the Russian Federation, Volgograd, Russia

    A. V. Strygin, V. I. Petrov, B. E. Tolkachev, E. I. Morkovin & A. O. Strygina

  2. Volgograd Medical Research Center, Volgograd, Russia

    A. V. Strygin, V. I. Petrov, B. E. Tolkachev, E. I. Morkovin & A. O. Strygina

  3. University Clinic of Medical Research and Educational Centre, M. V. Lomonosov Moscow State University, Moscow, Russia

    P. P. Nesmiyanov

  4. V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    P. P. Nesmiyanov

  5. Children’s Municipal Outpatient Clinic No. 86, Moscow Healthcare Department, Moscow, Russia

    M. V. Gutov

Authors
  1. A. V. Strygin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. P. Nesmiyanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. I. Petrov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. E. Tolkachev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. I. Morkovin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. V. Gutov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. O. Strygina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Strygin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 8, pp. 196-200, August, 2020

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Strygin, A.V., Nesmiyanov, P.P., Petrov, V.I. et al. Mycobacterium vaccae Lysate Induces Anti-Allergic Immune Response In Vitro. Bull Exp Biol Med 170, 226–229 (2020). https://doi.org/10.1007/s10517-020-05039-6

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-020-05039-6

Key Words

Search

Navigation