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Effect of Erythropoietin on Morphofunctional Properties of Mesenchymal Stem Cells

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of erythropoietin on the morphofunctional status of bone marrow mesenchymal stem cells in patients with coronary heart disease. It was shown that the duration of cell exposure with erythropoietin had different effects on the expression levels of adhesion molecules, erythropoietin receptors, and co-expression of the erythropoietin receptor and common β-chain of cytokines, apoptosis/necrosis, and the cell cycle. In most cases, erythropoietin increased proliferation, migration, and NO production by “aged” mesenchymal stem cells (passage 8) and passage 4 mesenchymal stem cells grown during the previous 3 passages in the presence of 33.4 U/ml erythropoietin. Erythropoietin increased the expression of the autophagy marker LC3B in mesenchymal stem cells grown in the presence of erythropoietin in the culture medium. Thus, long-term culturing of mesenchymal stem cells in the presence of erythropoietin in the culture medium increased their resistance to adverse microenvironment factors — oxidative stress and hyperglycemia.

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References

  1. Bondarenko NA, Lykov AP, Kazakov OV, Kabakov AV, Poveschenko OV, Surovtseva MA, Kim II, Poveschenko AF. Changes in functional properties of mesenchymal stem cells under the influence of erythropoietin. Sovremen. Problemy Nauki Obrazovaniya. 2017;(6):88-95. Russian.

  2. Gybskii YuI. Cell Death: Free Radicals, Necrosis, Apoptosis. Vinnitsa, 2015. Russian.

  3. Efimenko AY, Starostina EE, Rubina KA, Kalinina NI, Parfenova EV. Viability and angiogenic activity of mesenchymal stromal cells from adipose tissue and bone marrow under hypoxia and inflammation in vitro. Cell Tissue Biol. 2010;4(2):117-127.

  4. Zakharov YuM. Cytoprotective effects of erythropoietin. Klin. Nefrol. 2009;(1):16-21. Russian.

  5. Lykov AP, Nikonorova YV, Bondarenko NA, Poveshchenko OV, Kim II, Poveshchenko AF, Konenkov VI. Proliferation, Migration, and Production of Nitric Oxide by Bone Marrow Multipotent Mesenchymal Stromal Cells from Wistar Rats in Hypoxia and Hyperglycemia. Bull. Exp. Biol. Med. 2015;159(4):443-445. doi: https://doi.org/10.1007/s10517-015-2986-6

    Article  CAS  PubMed  Google Scholar 

  6. Lykov AP, Surovtseva MA, Poveshchenko OV, Chernyavsky AM, Fomichev AV, Bondarenko NA, Kim II. Effect of erythropoietin on bone marrow mononuclear cells. Med. Immunol. 2020;22(1):135-142. Russian.

  7. Makarevich PI, Boldyreva MA, Dergilev KV, Gluhanyuk EV, Gallinger JO, Efimenko AYu, Tkachuk VA, Parfenova EV. Transplantation of cell sheets from adipose-derived mesenchymal stromal cells effectively induces angiogenesis in ischemic skeletal muscle. Geny Kletki. 2015;10(3):68-77. Russian.

  8. Anagnostou A, Lee ES, Kessimian N, Levinson R, Steiner M. Erythropoietin has a mitogenic and positive chemotactic effect on endothelial cells. Proc. Natl Acad. Sci. USA. 1990;87(15):5978-5982.

    Article  CAS  Google Scholar 

  9. Bennis Y, Sarlon-Bartoli G, Guillet B, Lucas L, Pellegrini L, Velly L, Blot-Chabaud M, Dignat-Georges F, Sabatier F, Pisano P. Priming of late endothelial progenitor cells with erythropoietin before transplantation requires the CD131 receptor subunit and enhances their angiogenic potential. J. Thromb. Haemost. 2012;10(9):1914-1928.

    Article  CAS  Google Scholar 

  10. Castino R, Isidoro C, Murphy D. Autophagy-dependent cell survival and cell death in an autosomal dominant familial neurohypophyseal diabetes insipidus in vitro model. FASEB J. 2005;19(8):1024-1026.

    Article  CAS  Google Scholar 

  11. Chen Y, Ma Y, Li N, Wang H, Chen B, Liang Z, Ren R, Lu D, Boey J, Armstrong DG, Deng W. Efficacy and long-term longitudinal follow-up of bone marrow mesenchymal cell transplantation therapy in a diabetic patient with recurrent lower limb bullosis diabeticorum. Stem Cell Res. Ther. 2018;9(1):99. doi: https://doi.org/10.1186/s13287-018-0854-9

  12. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwit E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.

    Article  CAS  Google Scholar 

  13. Freitag J, F J, Bates D, Boyd R, Hahne A, Wang Y, Cicuttini F, Huguenin L, Norsworthy C, Shah K. Adipose derived mesenchymal stem cell therapy in the treatment of isolated knee chondral lesions: design of a randomised controlled pilot study comparing arthroscopic microfracture versus arthroscopic microfracture combined with postoperative mesenchymal stem cell injections. BMJ Open. 2015;5(12):009332. doi: https://doi.org/10.1136/bmjopen-2015-009332

    Article  Google Scholar 

  14. Honczarenko M, Le Y, Swierkowski M, Ghiran I, Glodek AM, Silberstein LE. Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors. Stem Cells. 2006;24(4):1030-1041.

    Article  CAS  Google Scholar 

  15. Kamianowska M, Szczepański M, Skrzydlewska E. Effects of erythropoietin on ICAM-1 and PECAM-1 expressions on human umbilical vein endothelial cells subjected to oxidative stress. Cell Biochem. Funct. 2011;29(6):437-441.

    Article  CAS  Google Scholar 

  16. Khorraminejad-Shirazi M, Farahmandnia M, Kardeh B, Estedlal A, Kardeh S, Monabati A. Aging and stem cell therapy: AMPK as an applicable pharmacological target for rejuvenation of “aged” stem cells and achieving higher efficacy in stem cell therapy. Hematol. Oncol. Stem Cell Ther. 2018;11(4):189-194.

    Article  CAS  Google Scholar 

  17. Lykov A, Poveshchenko O, Surovtseva M, Cherniavsky A, Fomichev A. Therapeutic potential of autologous bone marrow mononuclear cells preconditioned with Erythropoietin implantation in laser channels in patients with Cardiac arteria disease. JIOMICS. 2019;9(1):258. doi: https://doi.org/10.5584/jiomics.v9i1.258

    Article  Google Scholar 

  18. Mohler ER 3rd, Lifeng Zhang, Medenilla E, Rogers W, French B, Bantly A, Moore JS, Yonghong Huan, Murashima M, Berns JS. Effect of darbepoetin alfa on endothelial progenitor cells and vascular reactivity in chronic kidney disease. Vasc. Med. 2011;16(3):183-189.

  19. Shirakabe A, Ikeda Y, Sciarretta S, Zablocki DK, Sadoshima J. Aging and autophagy in the heart. Circ. Res. 2016; 118(10):1563-1576.

    Article  CAS  Google Scholar 

  20. Turinetto V, Vitale E, Giachino C. Senescence in human mesenchymal stem cells: functional changes and implications in stem cell-based therapy. Int. J. Mol. Sci. 2016;17(7). pii: E1164. doi: https://doi.org/10.3390/ijms17071164

  21. Vázquez-Mellado MJ, Cortés-Ballinas LG, Blanco-Flores IC, Aguilar C, Vázquez-Gómez G, Rocha-Zavaleta L. Erythropoietin promotes expression of survivin via STAT3 activation and reduces sensitivity to cisplatin in cervical cancer cells. Oncol. Rep. 2019;41(2):1333-1341.

    PubMed  Google Scholar 

  22. Wang P, Xie ZD, Xie CN, Lin CW, Wang JL, Xuan LN, Zhang CW, Wang Y, Huang ZH, Teng HL. AMP-activated protein kinase-dependent induction of autophagy bey erythropoietin protects against spinal cord injury in rats. CNS Neurosci. Ther. 2018;24(12):1185-1195.

    Article  CAS  Google Scholar 

  23. Wang FW, Wang Z, Zhang YM, Du ZX, Zhang XL, Liu Q, Guo YJ, Li XG, Hao AJ. Protective effect of melatonin on bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis in vitro. J. Cell. Biochem. 2013;114(10):2346-2355.

    Article  CAS  Google Scholar 

  24. Yang YK, Ogando CR, Wang See C, Chang TY, Barabino GA. Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro. Stem Cell Res. Ther. 2018;9(1):131. doi: https://doi.org/10.1186/s13287-018-0876-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Research Institute for Clinical and Experimental Lymphology — Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia

    A. P. Lykov, M. A. Surovtseva, I. I. Kim, N. A. Bondarenko & O. V. Poveshchenko

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  1. A. P. Lykov
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  2. M. A. Surovtseva
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  3. I. I. Kim
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  4. N. A. Bondarenko
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  5. O. V. Poveshchenko
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Correspondence to A. P. Lykov.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 209-216, September, 2020

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Lykov, A.P., Surovtseva, M.A., Kim, I.I. et al. Effect of Erythropoietin on Morphofunctional Properties of Mesenchymal Stem Cells. Bull Exp Biol Med 170, 164–170 (2020). https://doi.org/10.1007/s10517-020-05024-z

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  • DOI: https://doi.org/10.1007/s10517-020-05024-z

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