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Effect of Graphene Oxide Nanoparticles on Differentiation of Myeloid Suppressor Cells

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We studied the effect of graphene oxide (GO) nanoparticles on differentiation of human myeloid suppressor cells (MDSC) in an in vitro system. Separated mononuclear cells of healthy donors were induced with cytokines (IL-6 and GM-CSF) into the MDSC phenotype (both polymorphonuclear (PMN-MDSC) and monocyte (M-MDSC) subsets of these cells were taken into account). Pegylated GO nanoparticles (GO-PEG; mean size 569±14 nm, PEG content ~20%) were used. GO-PEG in low concentrations (2.5 and 5 μg/ml) increased the percentage of MDSC in cultures, but reduced their content in high concentration (10 μg/ml). After exposure to GO-PEG (2.5 and 5 μg/ml), the MDSC content increased at the expense of M-MDSC, while the level of PMN-MDSC did not change. The decrease in MDSC levels after exposure to high doses of GO-PEG (10 μg/ml) was due to a decrease in PMN-MDSC. Thus, GO-PEG nanoparticles can oppositely regulate differentiation of MDSC by inhibiting or stimulating differentiation of these cells depending on the concentration.

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  1. Dasari Shareena TP, McShan D, Dasmahapatra AK, Tchounwou PB. A review on graphene-based nanomaterials in biomedical applications and risks in environment and health. Nanomicro Lett. 2018;10(3):53.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. De Cicco P, Ercolano G, Rubino V, Terrazzano G, Ruggiero G, Cirino G, Ianaro A. Modulation of the functions of myeloid-derived suppressor cells: a new strategy of hydrogen sulfide anti-cancer effects. Br. J. Pharmacol. 2020;177(4):884-897.

    Article  Google Scholar 

  3. de Melo-Diogo D, Lima-Sousa R, Alves CG, Costa EC, Louro RO, Correia IJ. Functionalization of graphene family nanomaterials for application in cancer therapy. Colloids Surf. B Biointerfaces. 2018;171:260-275.

    Article  Google Scholar 

  4. Draghiciu O, Lubbers J, Nijman HW, Daemen T. Myeloid derived suppressor cells-An overview of combat strategies to increase immunotherapy efficacy. Oncoimmunology. 2015;4(1):e954829.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Dudek I, Skoda M, Jarosz A, Szukiewicz D. The molecular influence of graphene and graphene oxide on the immune system under in vitro and in vivo conditions. Arch. Immunol. Ther. Exp. (Warsz). 2016;64(3):195-215.

    Article  CAS  Google Scholar 

  6. Feito MJ, Vila M, Matesanz MC, Linares J, Gonçalves G, Marques PA, Vallet-Regí M, Rojo JM, Portolés MT. In vitro evaluation of graphene oxide nanosheets on immune function. J. Colloid Interface Sci. 2014;432:221-228.

    Article  CAS  Google Scholar 

  7. Gabrilovich DI, Nagaraj S. Myeloid derived-suppressor cells as regulators of the immune system. Nat. Rev. Immunol. 2009;9(3):162-174.

    Article  CAS  Google Scholar 

  8. Gabrilovich DI, Ostrand-Rosenberg S, Bronte V. Coordinated regulation of myeloid cells by tumours. Nat. Rev. Immunol. 2012;12(4):253-268.

    Article  CAS  Google Scholar 

  9. Greten TF, Manns MP, Korangy F. Myeloid Derived Suppressor Cells in Human Diseases. Int. Immunopharmacol. 2011;11(7):802-807.

    Article  CAS  Google Scholar 

  10. Hermanson GT. Bioconjugate Techniques. San Diego, 2008.

  11. Kumar V, Patel S, Tcyganov E, Gabrilovich DI. The nature of myeloid-derived suppressor cells in the tumor microenvironment. Trends Immunol. 2016;37(3):208-220.

    Article  CAS  Google Scholar 

  12. Lechner MG, Liebertz DJ, Epstein AL. Characterization of cytokine induced myeloid derived suppressor cells from normal human peripheral blood mononuclear cells. J. Immunol. 2010;185(4):2273-2284.

    Article  CAS  Google Scholar 

  13. Singh DP, Herrera CE, Singh B, Singh S, Singh RK, Kumar R. Graphene oxide: an efficient material and recent approach for biotechnological and biomedical applications. Mater. Sci. Eng. C Mater. Biol. Appl. 2018;86:173-197.

    Article  CAS  Google Scholar 

  14. Xu L, Xiang J, Liu Y, Xu J, Luo Y, Feng L, Liu Z, Peng R. Functionalized graphene oxide serves as a novel vaccine nano-adjuvant for robust stimulation of cellular immunity. Nanoscale. 2016;8(6):3785-3795.

    Article  CAS  Google Scholar 

  15. Xu M, Zhu J, Wang F, Xiong Y, Wu Y, Wang Q, Weng J, Zhang Z, Chen W, Liu S. Improved in vitro and in vivo biocompatibility of graphene oxide through surface modification: poly(acrylic Acid)-Functionalization is Superior to PEGylation. ACS Nano. 2016;10(3):3267-3281.

    Article  CAS  Google Scholar 

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Correspondence to S. A. Zamorina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 102-105, July, 2020

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Zamorina, S.A., Shardina, K.Y., Timganova, V.P. et al. Effect of Graphene Oxide Nanoparticles on Differentiation of Myeloid Suppressor Cells. Bull Exp Biol Med 170, 84–87 (2020).

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