Molecular and Genetic Bases of Inhibition of Tick-Borne Encephalitis Virus Replication by Eprosartan and Ribavirin

The antiviral activity of eprosartan (compound selected in silico) towards highly and low-virulent strains of tick-borne encephalitis virus was compared in vitro with activity of ribavirin. Study of the cytopathogenic activity of the virus on SPEV cells by ELISA, IFAT, and PCR showed similar results: both substances (eprosartan and ribavirin) promoted elimination of tick-borne encephalitis virus. Ribavirin exhibited intracellular inhibition towards both strains: the selectivity index for highly virulent Dal’negorsk strain was 160, for low-virulent Primorye-437 strain — 113. Eprosartan inhibited intracellular replication of Dal’negorsk strain (13.7) and less so that of Primorye-437 strain (2.9). The efficiency of virtual screening of the ligand (eprosartan) was demonstrated for highly virulent, but not low virulent tick-borne encephalitis strain.

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Correspondence to G. N. Leonova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 66-71, July, 2020

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Leonova, G.N., Maistrovskaya, O.S. & Lubova, V.A. Molecular and Genetic Bases of Inhibition of Tick-Borne Encephalitis Virus Replication by Eprosartan and Ribavirin. Bull Exp Biol Med 170, 53–57 (2020).

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Key Words

  • tick-borne encephalitis virus (TBE)
  • ribavirin
  • eprosartan
  • mechanisms of virus inhibition