The antiviral activity of eprosartan (compound selected in silico) towards highly and low-virulent strains of tick-borne encephalitis virus was compared in vitro with activity of ribavirin. Study of the cytopathogenic activity of the virus on SPEV cells by ELISA, IFAT, and PCR showed similar results: both substances (eprosartan and ribavirin) promoted elimination of tick-borne encephalitis virus. Ribavirin exhibited intracellular inhibition towards both strains: the selectivity index for highly virulent Dal’negorsk strain was 160, for low-virulent Primorye-437 strain — 113. Eprosartan inhibited intracellular replication of Dal’negorsk strain (13.7) and less so that of Primorye-437 strain (2.9). The efficiency of virtual screening of the ligand (eprosartan) was demonstrated for highly virulent, but not low virulent tick-borne encephalitis strain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson JF, Rahal JJ. Efficacy of interferon alpha-2b and ribavirin against West Nile virus in vitro. Emerg. Infect. Dis. 2002;8(1):107-108.
Belikov SI, Kondratov IG, Potapova UV, Leonova GN. The relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties. PLoS One. 2014;9(4):e94946. https://doi.org/10.1371/journal.pone.0094946
Crance JM, Scaramozzino N, Jouan A, Garin D. Interferon, ribavirin, 6-azauridine and glycyrrhizin: antiviral compounds active against pathogenic flaviviruses. Antiviral Res. 2003;58(1):73-79.
Dixit NM, Perelson AS. The metabolism, pharmacokinetics and mechanisms of antiviral activity of ribavirin against hepatitis C virus. Cell. Mol. Life Sci. 2006;63(7-8):832-842.
Feranchuk S, Potapova U, Belikov S. Virtual screening of inhibitors for the Zika virus proteins. bioRxiv. 2016. https://doi.org/10.1101/060798
Leonova GN, Belikov SI, Kondratov IG, Takashima I. Comprehensive assessment of the genetics and virulence of tickborne encephalitis virus strains isolated from patients with inapparent and clinical forms of the infection in the Russian Far East. Virology. 2013;443(1):89-98.
Leonova GN, Maystrovskaya OS, Kondratov IG, Takashima I, Belikov SI. The nature of replication of tick-borne encephalitis virus strains isolated from residents of the Russian Far East with inapparent and clinical forms of infection. Virus Res. 2014;189:34-42.
Leonova GN, Shutikova AL, Lubova VA, Maistrovskaya OS. Inhibitory activity of Scutellaria baicalensis flavonoids against tick-borne encephalitis virus. Bull. Exp. Biol. Med. 2020;168(5):665-668.
Potapova UV, Feranchuk SI, Potapov VV, Kulakova NV, Kondratov IG, Leonova GN, Belikov SI. NS2B/NS3 protease: allosteric effect of mutations associated with the pathogenicity of tick-borne encephalitis virus. J. Biomol. Struct. Dyn. 2012;30(6):638-651.
Shiryaev SA, Cheltsov AV, Gawlik K, Ratnikov BI, Strongin AY. Virtual ligand screening of the National Cancer Institute (NCI) compound library leads to the allosteric inhibitory scaffolds of the West Nile Virus NS3 proteinase. Assay Drug Dev. Technol. 2011;9(1):69-78.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 66-71, July, 2020
Rights and permissions
About this article
Cite this article
Leonova, G.N., Maistrovskaya, O.S. & Lubova, V.A. Molecular and Genetic Bases of Inhibition of Tick-Borne Encephalitis Virus Replication by Eprosartan and Ribavirin. Bull Exp Biol Med 170, 53–57 (2020). https://doi.org/10.1007/s10517-020-05003-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-020-05003-4