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Combined Administration of TLR4 (LPS) and TLR3 (Poly I:C) Ligands to CBA Mice Elevates the Content of Osteogenic MSC by 1.6 Times and Increases Content of Bone Marrow MSC to Intermediate Level between Values Attained by Their Individual Administration

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Bulletin of Experimental Biology and Medicine Aims and scope

In 1 and 24 h after combined administration of TLR4 (LPS) and TLR3 (Poly I:C) ligands to CBA mice, the content of MSC in bone marrow increased to intermediate value between the levels attained by their individual injections. The content of osteogenic MSC assessed in 24 h postinjection corresponded to the control level in Poly I:C group, decreased in LPS group by 2.5 times relatively to the control, and increased by 1.6 times (relatively to control) after combined administration of the ligands. In 3 h after combined addition of LPS and Poly I:C in vitro to 12-day-old primary culture of mouse bone marrow stromal cells, the concentration of TNFα in culture medium was intermediate between the levels attained by their individual application. The data revealed dependence of activation of stromal tissue on intensity of innate immunity reactions; they also attested to marked elevation of osteogenicity of MSC pool after costimulation with Poly I:C and LPS, which can underlie augmented calcification of the tissues during combined viral and bacterial infections.

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References

  1. Gorskaya YF, Danilova TA, Grabko VI, Nesterenko VG. Successive Administration of Streptococcus Type 5 Group A Antigens and S. typhimurium Antigenic Complex Corrects Elevation of Serum Cytokine Concentration and Number of Bone Marrow Stromal Pluripotent Cells in CBA Mice Induced by Each Antigen Separately. Bull. Exp. Biol. Med. 2015;160(2):256-259. https://doi.org/10.1007/s10517-015-3143-y

  2. Gorskaya YF, Danilova TA, Karyagina AS, Lunin VG, Grabko VI, Bartov MS, Gromov AV, Grunina TM, Soboleva LA, Shapoval IM, Nesterenko VG. Effects of combined treatment with complex S. typhimurium antigens and factors stimulating osteogenesis (curettage, BMP-2) on multipotent bone marrow stromal cells and serum concentration of cytokines in CBA mice. Bull. Exp. Biol. Med. 2015;158(4):465-470. https://doi.org/10.1007/s10517-015-2786-z

  3. Gorskaya YF, Nesterenko VG. The Content of Multipotent Stromal Cells in 3-4.5-Month Heterotopic Bone Marrow Transplants of CBA Mice Subjected to a Single Exposure to Osteogenic Stimuli (Curettage, BMP-2) or Antigens (S. typhimurium antigenic complex, LPS). Bull. Exp. Biol. Med. 2017;163(1):27-31. https://doi.org/10.1007/s10517-017-3754-6

  4. Gorskaya YF, Tukhvatulin AI, Nesterenko VG. NLR2 and TLR3, TLR4, TLR5 Ligands, Injected In Vivo, Improve after 1 h the Efficiency of Cloning and Proliferative Activity of Bone Marrow Multipotent Stromal Cells and Reduce the Content of Osteogenic Multipotent Stromal Cells in CBA Mice. Bull. Exp. Biol. Med. 2017;163(3):356-360. https://doi.org/10.1007/s10517-017-3803-1

    Article  CAS  PubMed  Google Scholar 

  5. Gorskaya YF, Tukhvatulin AI, Dzharullaeva AS, Nesterenko VG. Ligands of NOD2 (Muramyl Dipeptide) and TLR4 (LPS) in 24 h after Combined In Vivo Administration Produce a Synergistic Increase in the Content of Multipotent Stromal Cells in the Bone Marrow and Peritoneal Exudate of CBA Mice. Bull. Exp. Biol. Med. 2019;166(4):473-476. https://doi.org/10.1007/s10517-019-04375-6

    Article  CAS  PubMed  Google Scholar 

  6. Gorskaya YF, Tukhvatulin AI, Dzharullaeva AS, Semenova EN, Nagurskaya EV, Bekhalo VA, Nesterenko VG. Effect of Activated Immunocompetent Cells on the Number of Multipotent Stromal Cells in Bone Marrow Transplants of CBA and CBA/N Mice in a Short Time after Polyvinylpyrrolidone Administration to Animals. Bull. Exp. Biol. Med. 2019;166(3):348-352. https://doi.org/10.1007/s10517-019-04348-9

    Article  CAS  PubMed  Google Scholar 

  7. Deriu E, Boxx GM, He X, Pan C, Benavidez SD, Cen L, Rozengurt N, Shi W, Cheng G. Influenza virus affects intestinal microbiota and secondary salmonella infection in the gut through type I interferons. PLoS Pathog. 2016;12. N 5. ID e1005572. https://doi.org/10.1371/journal.ppat.1005572

  8. Jiang W, Sun R, Wei H, Tian Z. Toll-like receptor 3 ligand attenuates LPS-induced liver injury by down-regulation of toll-like receptor 4 expression on macrophages. Proc. Natl Acad. Sci. USA. 2005;102(47):17 077-17 082.

  9. Kim YG, Park JH, Shaw MH, Franchi L, Inohara N, Núñez G. The cytosolic sensors Nod1 and Nod2 are critical for bacterial recognition and host defense after exposure to Toll-like receptor ligands. Immunity. 2008;28(2):246-257.

    Article  CAS  Google Scholar 

  10. Marks LR, Davidson BA, Knight PR, Hakansson AP. Inter-kingdom signaling induces Streptococcus pneumoniae biofilm dispersion and transition from asymptomatic colonization to disease. MBio. 2013;4(4). https://doi.org/10.1128/mBio.00438-13

  11. Najar M, Krayem M, Meuleman N, Bron D, Lagneaux L. Mesenchymal stromal cells and Toll-like receptor priming: a critical review. Immune Netw. 2017;17(2):89-102.

    Article  Google Scholar 

  12. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, Cohen I.R, Zipori D. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109(4):1422-1432.

  13. Rashedi I, Gómez-Aristizábal A, Wang XH, Viswanathan S, Keating A. TLR3 or TLR4 activation enhances mesenchymal stromal cell-mediated Treg induction via Notch signaling. Stem Cells. 2017;35(1):265-275.

    Article  CAS  Google Scholar 

  14. Tukhvatulin AI, Gitlin II, Shcheblyakov DV, Artemicheva NM, Burdelya LG, Shmarov MM, Naroditsky BS, Gudkov AV, Gintsburg AL, Logunov DY. Combined stimulation of Toll-like receptor 5 and NOD1 strongly potentiates activity of NF-κB, resulting in enhanced innate immune reactions and resistance to Salmonella enterica serovar Typhimurium infection. Infect. Immun. 2013;81(10):3855-3864.

    Article  CAS  Google Scholar 

  15. van Heel DA, Ghosh S, Butler M, Hunt K, Foxwell BM, Mengin-Lecreulx D, Playford RJ. Synergistic enhancement of Toll-like receptor responses by NOD1 activation. Eur. J. Immunol. 2005;35(8):2471-2476.

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Immunity Regulation and Immunological Tolerance, Moscow, Russia

    Yu. F. Gorskaya & V. G. Nesterenko

  2. Laboratory of Cell Microbiology, N. F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia

    A. I. Tukhvatulin & A. Sh. Dzharullaeva

Authors
  1. Yu. F. Gorskaya
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  2. A. I. Tukhvatulin
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  3. A. Sh. Dzharullaeva
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  4. V. G. Nesterenko
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Corresponding author

Correspondence to Yu. F. Gorskaya.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 12, pp. 728-733, December, 2019

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Gorskaya, Y.F., Tukhvatulin, A.I., Dzharullaeva, A.S. et al. Combined Administration of TLR4 (LPS) and TLR3 (Poly I:C) Ligands to CBA Mice Elevates the Content of Osteogenic MSC by 1.6 Times and Increases Content of Bone Marrow MSC to Intermediate Level between Values Attained by Their Individual Administration. Bull Exp Biol Med 168, 767–772 (2020). https://doi.org/10.1007/s10517-020-04798-6

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  • DOI: https://doi.org/10.1007/s10517-020-04798-6

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