We studied the effect of unstable ubiquitous endocrine disruptors (bisphenol A and triclosan) on the reproductive function of mature male Wistar rats. Animals of both experimental groups received bisphenol A and triclosan (homogeneity>97%) in a dose of 200 mg/kg daily for 2 months with food. The concentrations of disruptors were measured directly in the testicular tissue by gas chromatography with mass spectrometry (GC-MS). In both experimental groups, the concentrations of bisphenol A and triclosan in the testicular tissue significantly differed from the control (intact animals). Animals exposed to bisphenol A significantly differed by sperm parameters (total number, motility, and sperm morphology) from control rats. Animals receiving triclosan significantly differed from control rats only by the number of actively motile spermatozoa and the weight of the testicles.
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Gromenko DS, Galimov ShN, Amirova ZK, Abdullina AZ, Gromenko YY, Galimova EF. Gonadotoxic effect of polychlorobiphenyls. Bull. Exp. Biol. Med. 2008;146(1):70-72.
Lutskii DL, Nikolaev AA. Morphological Examination of the Ejaculate. Astrakhan, 1999. Russian.
Tiktinskii OL. Andrology. St. Petersburg, 1999. Russian.
Chigrinets SV, Bryuhin GV, Ilenkho OS. Environmental exposure to endocrine disruptor of triclosan and semen quality of men. Probl. Reprod. 2018;24(3):61-66. Russian.
Andrology: Male Reproductive Health and Dysfunction. Nieschlag E, Behre HM, eds. Berlin, 2010.
Björndahl L, Söderlund I, Johansson S, Mohammadieh M, Pourian MR, Kvist U. Why the WHO recommendations for eosin-nigrosin staining techniques for human sperm vitality assessment must change. J. Androl. 2004;25(5):671-678.
Bryukhin GV, Sizonenko ML. Characteristic of sperm motility in mature offspring of females with experimental liver diseases of various origins. Bull. Exp. Biol. Med. 2014;158(1):27-29.
Den Hond E, Tournaye H, De Sutter P, Ombelet W, Baeyens W, Covaci A, Cox B, Nawrot T.S, Van Larebeke N, D’Hooghe T. Human exposure to endocrine disrupting chemicals and fertility: a case-control study in male subfertility patients. Environ. Int. 2015;84:154-160.
Kourouma A, Peng D, Chao Q, Changjiang L, Chengmin W, Wenjuan F, Suqin Q, Tingting Y, Kedi Y. Bisphenol A induced reactive oxygen species (ROS) in the liver and affect epididymal semen quality in adults Sprague-Dawley rats. J Toxicol. Environ. Health Sci. 2014;6(4):103-112.
Lan Z, Hyung Kim T, Shun Bi K, Hui Chen X, Sik Kim H. Triclosan exhibits a tendency to accumulate in the epididymis and shows sperm toxicity in male Sprague-Dawley rats. Environ. Toxicol. 2015;30(1):83-91.
Pernoncini KV, Montagnini BG, de Góes MLM, Garcia PC, Gerardin DCC. Evaluation of reproductive toxicity in rats treated with triclosan. Reprod. Toxicol. 2018;75:65-72.
Pirard C, Sagot C, Deville M, Dubois N, Charlier C. Urinary levels of bisphenol A, triclosan and 4-nonylphenol in a general Belgian population. Environ. Int. 2012;48):78-83.
Qiu LL, Wang X, Zhang XH, Zhang Z, Gu J, Liu L, Wang Y, Wang X, Wang SL. Decreased androgen receptor expression may contribute to spermatogenesis failure in rats exposed to low concentration of bisphenol A. Toxicol. Lett. 2013;219(2):116-124.
Wisniewski P, Romano RM, Kizys MM, Oliveira KC, Kasamatsu T, Giannocco G, Chiamolera MI, Dias-da-Silva M.R, Romano M.A. Adult exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis. Toxicology. 2015;329:1-9.
Wyrobek AJ, Bruce WR. Chemical induction of sperm abnormalities in mice. Proc. Natl Acad. Sci. USA. 1975;72(11):4425-4429.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 12, pp. 712-716, December, 2019
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Chigrinets, S.V., Bryuhin, G.V. & Zavyalov, S.N. Characterization of Sperm of White Rats at Exposure of Bisphenol A and Triclosan. Bull Exp Biol Med 168, 753–756 (2020). https://doi.org/10.1007/s10517-020-04795-9
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DOI: https://doi.org/10.1007/s10517-020-04795-9