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Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus

We studied virus-inhibiting activity of Baikal skullcap (Scutellaria baicalensis) flavonoids against tick-borne encephalitis virus using various model schemes. The half-maximum cytotoxic concentration (CC50) for the plant extract was found (363.9±58.6 μg/ml). Based on the CC50 and IC50, selective index (SI) was calculated for viricidal (53.4), preventive (50.5), and direct antiviral actions (39.1) and for-intracellular replication of the virus (40.4). Suppression of virus reproduction ≥2.0 lg TCID50 was observed at extract concentration ≥5 μg/ml (viricidal effect), ≥11.2 μg/ml (preventive and direct antiviral effects), and ≥9 μg/ml (intracellular replication). Flavonoids of Baikal skullcap extract produced an in vitro inhibitory effect on tick-borne encephalitis virus due to their direct viricidal activity and direct inhibition of adsorption and intracellular replication of tick-borne encephalitis virus, which determines their value as highly effective antiviral drugs.

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Correspondence to G. N. Leonova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 11, pp. 611-614, November, 2019

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Leonova, G.N., Shutikova, A.L., Lubova, V.A. et al. Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus. Bull Exp Biol Med 168, 665–668 (2020).

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  • tick-borne encephalitis virus (TBEV)
  • inhibition
  • Baikal skullcap