Skip to main content

Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus

Bulletin of Experimental Biology and Medicine volume 168, pages 665–668 (2020)Cite this article

We studied virus-inhibiting activity of Baikal skullcap (Scutellaria baicalensis) flavonoids against tick-borne encephalitis virus using various model schemes. The half-maximum cytotoxic concentration (CC50) for the plant extract was found (363.9±58.6 μg/ml). Based on the CC50 and IC50, selective index (SI) was calculated for viricidal (53.4), preventive (50.5), and direct antiviral actions (39.1) and for-intracellular replication of the virus (40.4). Suppression of virus reproduction ≥2.0 lg TCID50 was observed at extract concentration ≥5 μg/ml (viricidal effect), ≥11.2 μg/ml (preventive and direct antiviral effects), and ≥9 μg/ml (intracellular replication). Flavonoids of Baikal skullcap extract produced an in vitro inhibitory effect on tick-borne encephalitis virus due to their direct viricidal activity and direct inhibition of adsorption and intracellular replication of tick-borne encephalitis virus, which determines their value as highly effective antiviral drugs.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. Belikov SI, Kondratov IG, Potapova UV, Leonova GN. The relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties. PLoS One. 2014;9(4). ID e94946. doi: https://doi.org/10.1371/journal.pone.0094946

  2. Chen L, Dou J, Su Z, Zhou H, Wang H, Zhou W, Guo Q, Zhou C. Synergistic activity of baicalein with ribavirin against influenza A (H1N1) virus infections in cell culture and in mice. Antiviral Res. 2011;91(3):314-320.

    Article  CAS  Google Scholar 

  3. Dou J, Chen L, Xu G, Zhang L, Zhou H, Wang H, Su Z, Ke M, Guo Q, Zhou C. Effects of baicalein on Sendai virus in vivo are linked to serum baicalin and its inhibition of hemagglutinin-neuraminidase. Arch. Virol. 2011;156(5):793-801.

    Article  CAS  Google Scholar 

  4. Hudson JB. Applications of the phytomedicine Echinacea purpurea (purple coneflower) in infectious diseases. J. Biomed. Biotechnol. 2012;2012. ID 769896. doi: https://doi.org/10.1155/2012/769896

  5. Johari J, Kianmehr A, Mustafa MR, Abubakar S, Zandi K. Antiviral activity of baicalein and quercetin against the Japanese encephalitis virus. Int. J. Mol. Sci. 2012;13(12):16,785-16,795.

    Article  CAS  Google Scholar 

  6. Lai MY, Hsiu SL, Chen CC, Hou YC, Chao PD. Urinary pharmacokinetics of baicalein, wogonin and their glycosides after oral administration of Scutellariae Radix in humans. Biol. Pharm. Bull. 2013;26(1):79-83.

    Article  Google Scholar 

  7. Leonova GN, Belikov SI, Kondratov IG, Takashima I. Comprehensive assessment of the genetics and virulence of tick-borne encephalitis virus strains isolated from patients with inapparent and clinical forms of the infection in the Russian Far East. Virology. 2013;443(1):89-98.

    Article  CAS  Google Scholar 

  8. Li HB, Chen F. Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant S. baicalensis by high-speed counter-current chromatography. J. Chromatogr. A. 2005;1074(1-2):107-110.

    Article  CAS  Google Scholar 

  9. Oo A, Teoh BT, Sam SS, Bakar SA, Zandi K. Baicalein and baicalin as Zika virus inhibitors. Arch. Virol. 2019;164(2):585-593.

    Article  CAS  Google Scholar 

  10. Scheck AC, Perry K, Hank NC, Clark WD. Anticancer activity of extracts derived from the mature roots of S. baicalensis on human malignant brain tumor cells. BMC Complement. Altern. Med. 2006;16:27.

  11. Xu G, Dou J, Zhang L, Guo Q, Zhou C. Inhibitory effects of baicalein on the Influenza virus in vivo is determined by baicalin in the serum. Biol. Pharm. Bull. 2010;33(2):238-243.

    Article  CAS  Google Scholar 

  12. Yang Y, Zhang L, Fan X, Qin C, Liu J. Antiviral effect of geraniin on human enterovirus 71 in vitroand in vivo. Bioorg. Med. Chem. Lett. 2012;22(6):2209-2211.

    Article  CAS  Google Scholar 

  13. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, Abubakar S. Novel antiviral activity of baicalein against dengue virus. BMC Complement. Altern. Med. 2012;12. ID 214. doi: https://doi.org/10.1186/1472-6882-12-214

  14. Zhang N, Van Crombruggen K, Holtappels G, Bachert C. A herbal composition of Scutellaria baicalensis and Eleutherococcus senticosus shows potent anti-inflammatory effects in an ex vivo human mucosal tissue model. Evid. Based Complement. Alternat. Med. 2012;2012. ID 673145. doi: https://doi.org/10.1155/2012/673145

Download references

Author information

Authors and Affiliations

  1. G. P. Somov Research Institute of Epidemiology and Microbiology, Vladivostok, Russia

    G. N. Leonova, A. L. Shutikova, V. A. Lubova & O. S. Maistrovskaya

Authors
  1. G. N. Leonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. L. Shutikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. A. Lubova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. S. Maistrovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. N. Leonova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 11, pp. 611-614, November, 2019

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Leonova, G.N., Shutikova, A.L., Lubova, V.A. et al. Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus. Bull Exp Biol Med 168, 665–668 (2020). https://doi.org/10.1007/s10517-020-04776-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-020-04776-y

Keywords

  • tick-borne encephalitis virus (TBEV)
  • inhibition
  • Baikal skullcap