We studied the effect of a new cyanine dye containing selenium and tellurium on acetylcholinesterase activity in synaptic membrane in rat brain. The cyanine dye dose-dependently inhibits activity of this enzyme, and the concentration of half-maximal inhibition of acetylcholinesterase activity was 20.46 μM. The cyanine dye instantly inhibits the enzyme; the degree of inhibition depends on acetylthiocholine concentration: the lower is acetylthiocholine concentration, the higher is the degree of inhibition. On the Lineweaver—Burk plot, the concentration dependence curves of acetylcholinesterase with and without cyanine dye intersect in one point on the abscissa axis. In this case, the cyanine dye reduces the maximum inhibition rate (Vmax) and does not affect Michaelis constant (Km). The calculated inhibition constant Ki for the cyanine dye is 7.74 μM. Thus, the cyanine dye is a non-competitive inhibitor of acetylcholinesterase.
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References
Alzheimer’s Association. 2013 Alzheimer’s disease facts and figures. Alzheimers Dement. 2013;9(2):208-245.
Cacabelos R, Torrellas C, Teijido O, Carril JC. Pharmacogenetic considerations in the treatment of Alzheimer’s disease. Pharmacogenomics. 2016;17(9):1041-1074.
Fernández-Lodeiro J, Pinatto-Botelho MF, Soares-Paulino AA, Gonçalves AC, Sousa BA, Princival C, Dos Santos AA. Synthesis and biological properties of selenium- and tellurium-containing dyes. Dyes and Pigments. 2014;110:28-48.
Hicks D, John D, Makova NZ, Henderson Z, Nalivaeva NN, Turner AJ. Membrane targeting, shedding and protein interactions of brain acetylcholinesterase. J. Neurochem. 2011;116(5):742-746.
Johnson JL, Cusack B, Davies MP, Fauq A, Rosenberry TL. Unmasking tandem site interaction in human acetylcholinesterase. Substrate activation with a cationic acetanilide substrate. Biochemistry. 2003;42(18):5438-5452.
Lendvai B, Vizi ES. Nonsynaptic chemical transmission through nicotinic acetylcholine receptors. Physiol. Rev. 2008; 88(2):333-349.
Rahim F, Javed MT, Ullah H, Wadood A, Taha M, Ashraf M, Qurat-ul-Ain, Khan MA, Khan F, Mirza S, Khan KM. Synthesis, molecular docking, acetylcholinesterase and butyrylcholinesterase inhibitory potential of thiazole analogs as new inhibitors for Alzheimer disease. Bioorg. Chem. 2015; 62):106-116.
Singh A. Alzheimer’s disease Inhibitors: Current status and future prospects. Int. J. Pharm. Life Sci. 2014;5(8):3734-3740.
Zheng J, He M, Xie B, Yang L, Hu Z, Zhou HB, Dong C. Enantioselective synthesis of novel pyrano[3,2-c]chromene derivatives as AChE inhibitors via an organocatalytic domino reaction. Org. Biomol. Chem. 2018;16(3):472-479.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 8, pp. 181-184, August, 2019
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Klichkhanov, N.K., Dzhafarova, A.M., Ramazanova, P.A. et al. A New Tellurium- and Selenoorganic Compound as an Inhibitor of Acetylcholinesterase in Brain. Bull Exp Biol Med 168, 229–232 (2019). https://doi.org/10.1007/s10517-019-04680-0
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DOI: https://doi.org/10.1007/s10517-019-04680-0