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Effect of Bioactive Peptide Complex Isolated from Bovine Serum on Proliferation and Migration of Mesenchymal Stromal Cells In Vitro and Reparation of Bone Defects In Vivo

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We studied the effect of a bioactive peptide complex isolated from bovine serum on the proliferative potential and migration rate of mesenchymal stromal cells in vitro, as well as on the healing of modeled bone defects in rats. This bioregulatory preparation stimulated proliferation of mesenchymal stromal cells from deciduous tooth pulp in vitro, but did not affect the rate of their migration in two-dimensional cultures. In vivo experiments showed that application of this preparation in combination with hydroxyapatite and chitosan gel accelerated bone tissue regeneration, thus ensuring restoration of morphologically normal bone matrix. Thus, cattle blood serum is an available source for the production of bioregulatory preparations for medical purposes.

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Authors and Affiliations

  1. I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia

    A. I. Shaikhaliev

  2. A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia

    M. S. Krasnov, A. P. Il’ina & I. A. Yamskov

  3. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

    I. V. Vakhrushev & K. N. Yarygina

  4. N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    E. Yu. Rybakova & V. P. Yamskova

Authors
  1. A. I. Shaikhaliev
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  2. M. S. Krasnov
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  3. I. V. Vakhrushev
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  4. A. P. Il’ina
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  5. E. Yu. Rybakova
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  6. K. N. Yarygina
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  7. V. P. Yamskova
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  8. I. A. Yamskov
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Corresponding author

Correspondence to M. S. Krasnov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 213-220, September, 2019

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Shaikhaliev, A.I., Krasnov, M.S., Vakhrushev, I.V. et al. Effect of Bioactive Peptide Complex Isolated from Bovine Serum on Proliferation and Migration of Mesenchymal Stromal Cells In Vitro and Reparation of Bone Defects In Vivo. Bull Exp Biol Med 168, 178–185 (2019). https://doi.org/10.1007/s10517-019-04671-1

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  • DOI: https://doi.org/10.1007/s10517-019-04671-1

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