We developed a technological accessory bacteriophage-based preparation and a method for phage-mediated bioprocessing for elimination of pathogenic microorganisms from the surface of fresh fish and for prolongation of the shelf-life of chilled hydrobionts. Specimens of rainbow trout (Salmo irideus) served as the objects of the study carried out at a fish-processing plant in the Republic of Karelia. The specimens were decontaminated by a bacteriophage cocktail containing six original virulent phage strains characterized by their pheno- and genotypical properties. A new method of biodecontamination (plunging the rainbow trout for 30 sec into a solution of bacteriophage cocktail (bacteriophage titers ≥108 PFU/ml) delayed bacterial degradation of hydrobionts by 3 days. The use of the new method for decontamination of food half-products — phage-mediated bioprocessing — promoted preservation of the initial ecological purity, nutritive value, and taste of the products and prolonged their shelf-life in comparison with the actual standards.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aleshkin AV, Karaulov AV, Svetoch EA, Volozhantsev NV, Aleshkin VA, Afanasiev SS, Rubalskiy OV, Vasil’ev DA, Zolotuhin SN, Afanasiev MS, Lakhtin VM, Rubalskiy MO. Bacteriophages as probiotic tools to regulate microbiocenosis and decontaminate food products, animals and plants with microorganisms. Immunopatol., Allergol., Infektol. 2013;(3):80-89. Russian.
Kiseleva IA, Aleshkin AV, Verevkin VV, Svetoch EA, Afanas’ev SS, Rubal’skij EO, Rubal’skaja EE, Rubal’skij MO, Efimova OG, Vasil’ev DA, Zolotukhin SN. Patent RU No. 2525141. Method of production of bacteriophage. Bull. No. 22. Published August 10, 2014.
Manual for Preclinical Studies of New Pharmacological Substances. Mironov AN, ed. Moscow, 2013. Russian.
Alikhan NF, Petty NK, Ben Zakour NL, Beatson SA. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics. 2011;12. ID 402. doi: https://doi.org/10.1186/1471-2164-12-402
Beutin L. Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J. Vet. Med. B Infect. Dis. Vet. Public Health. 2006;53(7):299-305.
Crevel RW, Ballmer-Weber BK, Holzhauser T, Hourihane JO, Knulst AC, Mackie AR, Timmermans F, Taylor SL. Thresholds for food allergens and their value to different stakeholders. Allergy. 2008;63(5):597-609.
DuPont HL. The growing threat of foodborne bacterial enteropathogens of animal origin. Clin. Infect. Dis. 2007;45(10):1353-1361.
García P, Martínez B, Obeso JM, Rodríguez A. Bacteriophages and their application in food safety. Lett. Appl. Microbiol. 2008;47(6):479-485.
Golosova O, Henderson R, Vaskin Y, Gabrielian A, Grekhov G, Nagarajan V, Oler A.J, Quiñones M, Hurt D, Fursov M, Huyen Y. Unipro UGENE NGS pipelines and components for variant calling, RNA-seq and ChIP-seq data analyses. PeerJ. 2014;2. ID e644. doi: https://doi.org/10.7717/peerj.644
McNair K, Bailey BA, Edwards RA. PHACTS, a computational approach to classifying the lifestyle of phages. Bioinformatics. 2012;28(5):614-618.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 6, pp. 783-787, June, 2019
Rights and permissions
About this article
Cite this article
Zulkarneev, E.R., Aleshkin, A.V., Kiseleva, I.A. et al. Bacteriophage Cocktail Effectively Prolonging the Shelf-Life of Chilled Fish. Bull Exp Biol Med 167, 818–822 (2019). https://doi.org/10.1007/s10517-019-04630-w
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04630-w