We studied proliferative activity of colorectal cancer cells with different expression level of CD133 molecule associated with cancer stem cells phenotype. Analysis of BrdU incorporation into Caco-2 and HT-29 cell lines showed that the percentage of cells in the DNA synthesis phase in the CD133+/high population is higher than in CD133—/low population. The expression of proliferation marker Ki-67 and the percentage of Ki-67+ cells were also higher in the CD133+/high population. Colorimetric analysis with crystal violet dye showed that the number of cells after 10-days culturing was higher in the CD133+/high population in both cell lines. These findings suggest that cells with high level of CD133 expression are characterized by higher proliferative activity, which can contribute to the tumor progression.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 84-89, June, 2019
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Gisina, A.M., Kim, Y.S., Potashnikova, D.M. et al. Proliferative Activity of Colorectal Cancer Cells with Different Levels of CD133 Expression. Bull Exp Biol Med 167, 541–545 (2019). https://doi.org/10.1007/s10517-019-04569-y
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DOI: https://doi.org/10.1007/s10517-019-04569-y