The effect of H2S on changes in erythrocyte volume was studied by spectrophotometrical and potentiometric methods. It was found that H2S donor NaHS (2.5, 10, and 100 μM) induced an increase in erythrocyte volume in heterosmotic media. Activation of Gardos channels with A23187 or ascorbate—phenazine methosulfate system causes erythrocyte shrinkage and hyperpolarization of their membrane, while addition of NaHS restored erythrocyte volume. The decrease in erythrocyte volume upon blockade of Na+,K+,2Cl— cotransporter (bumetanide) or anion exchanger (SITS) was abolished by H2S donor NaHS, which attested to an important role of these transporters and chlorine conductivity of the membrane in the maintenance of the homeostasis of blood cells.
Similar content being viewed by others
References
Gabitova DM, Shaidullov IF, Sabirullina GI, Shafigullin MU, Sitdikov FG, Sitdikova GF. Role of Cyclic Nucleotides in the Effect of Hydrogen Sulfide on Contractions of Rat Jejunum. Bull. Exp. Biol. Med. 2017;163(1):14-17.
Gusakova SV, Kovalev IV, Birulina YG, Smagliy LV, Petrova IV, Nosarev AV, Orlov SN, Aleinyk AN. The effects of carbon monoxide and hydrogen sulfide on transmembrane ion transport. Biophysics. 2017;62(2):220-226.
Petrova IV, Trubacheva OA, Mangataeva OS, Suslova TE, Kovalev IV, Gusakova SV. The influence of hydrogen sulfide on collagen-induced aggregation of human platelets. Ross. Fiziol. Zh. 2015;101(10):1191-1201. Russian.
Andolfo I, Russo R, Gambale A, Iolascon A. New insights on hereditary erythrocyte membrane defects. Haematologica. 2016;101(11):1284-1294.
Bonar PT, Casey JR. Plasma membrane Cl—/HCO3— exchangers: structure, mechanism and physiology. Channels. 2008;2(5):337-345.
Kabil O, Banerjee R. Enzymology of H2S biogenesis, decay and signaling. Antioxid. Redox Signal. 2014;20(5):770-782.
Lang F. Mechanisms and significance of cell volume regulation. J. Am. Coll. Nutr. 2007;26(5 Suppl):613S-623S.
Maher AD, Kuchel PW. The Gаrdos channel: a review of the Ca2+-activated K+ channel in human erythrocytes. Int. J. Biochem. Cell Biol. 2003;35(8):1182-1197.
Polhemus DJ, Lefer DJ. Emergence of hydrogen sulfide as an endogenous gaseous signaling molecule in cardiovascular disease. Circ. Res. 2014;114(4):730-737.
Sprague RS, Stephenson AH, Ellsworth ML. Red not dead: signaling in and from erythrocytes. Trends Endocrinol. Metab. 2007;18:350-355.
Srinivas SP, Bonanno JA, Larivière E, Jans D, Van Driessche W. Measurement of rapid changes in cell volume by forward light scattering. Pflugers Arch. 2003;447(1):97-108.
Zhang W, Xu C, Yang G, Wu L, Wang R. Interaction of H2S with calcium permeable channels and transporters. Oxid. Med. Cell. Longev. 2015;2015. ID 323269. doi: https://doi.org/10.1155/2015/323269.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 4, pp. 497-500, April, 2019
Rights and permissions
About this article
Cite this article
Birulina, Y.G., Petrova, I.V., Rozenbaum, Y.A. et al. H2S-Mediated Changes in Erythrocyte Volume: Role of Gardos Channels, Na+,K+,2Cl— Cotransport and Anion Exchanger. Bull Exp Biol Med 167, 508–511 (2019). https://doi.org/10.1007/s10517-019-04561-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04561-6