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Role of α-Fetoprotein in Regulation of Proliferation and Functional Activity of Naïve T Cells and Immune Memory T Cells

  • IMMUNOLOGY AND MICROBIOLOGY
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We studied the role of native α-fetoprotein preparation in the regulation of proliferation and functional activity of naïve T cells and immune memory T cells in vitro. The study was carried out on separated fractions of naïve T cells (CD45RA+) and immune memory T cells (CD45R0+) incubated with α-fetoprotein under conditions of TCR activation. At the level of naïve T cells, α-fetoprotein in a concentration of 100 U/ml reduced the expression of CD28, but increased the expression of CD25, while at the level of immune memory T cells α-fetoprotein (50 and 100 U/ml) only suppressed the expression of CD25. No effects of α-fetoprotein on the proliferative status of the studied lymphocyte subpopulations and on the expression of CD71 (proliferation marker) by these cells were detected. Addition of α-fetoprotein in a concentration of 100 U/ml increased the level of IL-2 in naïve T cell culture supernatants, while production of IL-2 by memory T cells remained unchanged. These data demonstrated the priority aspects of regulation of the functional activities of naïve T cells and immune memory T cells.

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References

  1. Kudryavtsev IV. Memory T cells: major populations and stages of differentiation. Ross. Immunol. Zh. 2014;8(4):947-964. Russian.

    Google Scholar 

  2. Litvinova LS, Gutsol AA, Sokhonevich NA, Kofanova KA, Khaziakhmatova OG, Shupletsova VV, Kaigorodova EV, Goncharov AG. Basic surface markers of functional activity Tlymphocytes. Med. Immunol. 2014;16(1):7-26. Russian.

    Article  Google Scholar 

  3. Chereshnev VA, Timganova VP, Zamorina SA, Bochkova MS, Khramtsov PV, Kropaneva MD, Raev MB. Role of α-fetoprotein in differentiation of regulatory T lymphocytes. Dokl. Biol. Sci. 2017;477(1):248-251.

    Article  CAS  Google Scholar 

  4. Fettke F, Schumacher A, Canellada A, Toledo N, Bekeredjian- Ding I, Bondt A, Wuhrer M, Costa SD, Zenclussen AC. Maternal and fetal mechanisms of B cell regulation during pregnancy: human chorionic gonadotropin stimulates B cells to produce IL-10 while α-fetoprotein drives them into apoptosis. Front. Immunol. 2016;8(7). ID 495. doi: https://doi.org/10.3389/fimmu.2016.00495

  5. MacDonald HR, Zaech P. Light scatter analysis and sorting of cells activated in mixed leukocyte culture. Cytometry. 1982;3(1):55-58.

    Article  CAS  Google Scholar 

  6. McNeill L, Salmond RJ, Cooper JC, Carret CK, Cassady-Cain RL, Roche-Molina M, Tandon P, Holmes N, Alexander DR. The differential regulation of Lck kinase phosphorylation sites by CD45 is critical for T cell receptor signaling responses. Immunity. 2007;27(3):425-437.

    Article  CAS  Google Scholar 

  7. Schumacher A, Costa SD, Zenclussen AC. Endocrine factors modulating immune responses in pregnancy. Front. Immunol. 2014;5. ID 196. doi: https://doi.org/10.3389/fimmu.2014.00196

  8. Shipkova M, Wieland E. Surface markers of lymphocyte activation and markers of cell proliferation. Clin. Chim. Acta. 2012;413(17-18):1338-1349.

    Article  CAS  Google Scholar 

  9. Terentiev AA, Moldogazieva NT. Alpha-fetoprotein: a renaissance. Tumor Biol. 2013;34(4):2075-2091.

    Article  CAS  Google Scholar 

  10. Torres JM, Laborda J, Naval J, Darracq N, Calvo M, Mishal Z, Uriel J. Expression of alpha-fetoprotein receptors by human T-lymphocytes during blastic transformation. Mol. Immunol. 1989;26(9):851-857.

    Article  CAS  Google Scholar 

  11. Veselá R, Doležalová L, Pytlík R, Rychtrmocová H, Marečková H, Trněný M. The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incor-poration of (3) H-thymidine and differential gating method. Cell. Immunol. 2011;271(1):78-84. doi: https://doi.org/10.1016/j.cellimm.2011.06.006

    Article  PubMed  CAS  Google Scholar 

  12. Yamamoto M, Tatsumi T, Miyagi T, Tsunematsu H, Aketa H, Hosui A, Kanto T, Hiramatsu N, Hayashi N, Takehara T. α-Fetoprotein impairs activation of natural killer cells by inhibiting the function of dendritic cells. Clin. Exp. Immunol. 2011;165(2):211-219.

    Article  CAS  Google Scholar 

  13. Zamorina SA, Litvinova LS, Yurova KA, Khaziakhmatova OG, Timganova VP, Bochkova MS, Khramtsov PV, Rayev MB. The role of human chorionic gonadotropin in regulation of naïve and memory T cells activity in vitro. Int. Immunopharmacol. 2018;54:33-38.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, Perm, Russia

    S. A. Zamorina, V. P. Timganova, M. S. Bochkova, P. V. Khramtsov, M. B. Raev & V. A. Chereshnev

  2. I. Kant Baltic Federal University, Kaliningrad, Russia

    L. S. Litvinova, K. A. Yurova, O. G. Khaziakhmatova & P. V. Khramtsov

  3. Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Yekaterinburg, Russia

    V. A. Chereshnev

Authors
  1. S. A. Zamorina
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  2. L. S. Litvinova
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  3. K. A. Yurova
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  4. O. G. Khaziakhmatova
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  5. V. P. Timganova
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  6. M. S. Bochkova
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  7. P. V. Khramtsov
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  8. M. B. Raev
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  9. V. A. Chereshnev
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Corresponding author

Correspondence to S. A. Zamorina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 4, pp. 454-458, April, 2019

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Zamorina, S.A., Litvinova, L.S., Yurova, K.A. et al. Role of α-Fetoprotein in Regulation of Proliferation and Functional Activity of Naïve T Cells and Immune Memory T Cells. Bull Exp Biol Med 167, 470–474 (2019). https://doi.org/10.1007/s10517-019-04552-7

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  • DOI: https://doi.org/10.1007/s10517-019-04552-7

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