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High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology

  • BIOPHYSICS AND BIOCHEMISTRY
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Bulletin of Experimental Biology and Medicine Aims and scope

A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.

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Authors and Affiliations

  1. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    Ya. A. Lomakin, A. N. Kaminskaya, A. V. Stepanov, A. A. Shmidt, A. G. Gabibov & A. A. Belogurov Jr

  2. Kazan (Volga region) Federal University, Kazan, Republic of Tatarstan, Russia

    Ya. A. Lomakin & A. V. Stepanov

  3. M. V. Lomonosov Moscow State University, Moscow, Russia

    A. G. Gabibov & A. A. Belogurov Jr

Authors
  1. Ya. A. Lomakin
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  2. A. N. Kaminskaya
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  3. A. V. Stepanov
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  4. A. A. Shmidt
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  5. A. G. Gabibov
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  6. A. A. Belogurov Jr
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Corresponding author

Correspondence to Ya. A. Lomakin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 4, pp. 428-434, April, 2019

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Lomakin, Y.A., Kaminskaya, A.N., Stepanov, A.V. et al. High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology. Bull Exp Biol Med 167, 446–451 (2019). https://doi.org/10.1007/s10517-019-04546-5

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  • DOI: https://doi.org/10.1007/s10517-019-04546-5

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