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A Combination of Muramylpeptides from Gram-Negative Bacteria Corrects Hematological and Immunological Disorders Induced by Cyclophosphamide

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Bulletin of Experimental Biology and Medicine Aims and scope

We have studied the effect of a combination of three natural muramylpeptides containing a meso-diaminopimelic acid residue (polyramyl) on the subpopulations of circulating T cells, spleen morphology, and leukocyte level in the blood of C57Bl/6 mice with cyclophosphamideinduced immunosuppression. Intraperitoneal injections of cyclophosphamide in a dose of 100 mg/kg on days 1, 3, 5, and 7 of the experiment reduced leukocyte count and the relative number of CD4+ T cells in the blood, and also depleted the cellular composition of splenic white pulp on day 10. Subcutaneous injections of polyramyl in a dose of 200 μg/mouse on days 8 and 9 practically completely restored blood leukocytes count and morphology of the splenic white pulp. Moreover, administration of polyramyl induced marked tendency to increase in the relative number of CD4+ T cells and CD4/CD8 ratio in mice with cyclophosphamideinduced immunosuppression.

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References

  1. Karaulov AV, Kalyuzhin OV. Sphere of muramyl dipeptide application within the major approaches to immunotherapy/prophylaxis of infectious diseases. Fiziol. Patol. Immun. Systemy. 2013;17(5):3-15. Russian.

    Google Scholar 

  2. Lebedinskaya EA, Lebedinskaya OV, Godovalov AP, Prokudin VS. Immuno-morphological features of the rat’s thymus at the experimental administration of cytostatics. Saratovsk. Nauch.-Med. Zh. 2016;12(1):12-14. Russian.

    CAS  Google Scholar 

  3. Lebedinskaya EA, Loseva LF, Lebedinskaya OV, Akhmatova NK. Correction of changes in cytokine profile of mice with the induced immunosupression. Tsitokiny Vospalenie. 2011;10(3):108-112. Russian.

    Google Scholar 

  4. L’vov VL, Pinegin BV, Khaitov RM, Pashchenkov MV. Patent RU No. 2441906. Composition comprising low-molecular fragments of peptidoglicane of gram-negative bacteria for preventing or treating of human diseases. Bull. No. 4. Published February 10, 2012.

  5. Fukuoka M, Takada M, Takifuji N, Sakai N, Ryu S, Masuda N, Matsui K, Negoro S, Kusunoki Y, Tubura E. Restorative effect of muroctasin on leukopenia caused by anticancer chemotherapy in lung cancer. Comparative study by envelope method. Arzneimittelforschung. 1989;39(1):90-93.

    CAS  PubMed  Google Scholar 

  6. Kelleher FC, O’Sullivan H. Monocytes, Macrophages, and Osteoclasts in Osteosarcoma. J. Adolesc. Young Adult Oncol. 2017;6(3):396-405.

    Article  PubMed  Google Scholar 

  7. Meyers PA, Chou AJ. Muramyl tripeptide-phosphatidyl ethanolamine encapsulated in liposomes (L-MTP-PE) in the treatment of osteosarcoma. Adv. Exp. Med. Biol. 2014;804:307-321.

    Article  CAS  PubMed  Google Scholar 

  8. Roberts SS, Chou AJ, Cheung NK. Immunotherapy of Childhood Sarcomas. Front. Oncol. 2015;5:181. doi: https://doi.org/10.3389/fonc.2015.00181.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Wuest B, Wachsmuth E.D. Stimulatory effect of N-acetyl muramyl dipeptide in vivo: proliferation of bone marrow progenitor cells in mice. Infect. Immun. 1982;37(2):452-462.

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Cellular Immunity, N. N. Blokhin National Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia

    M. V. Kiselevskii, N. Yu. Anisimova, S. M. Sitdikova & F. V. Donenko

  2. CORUS Pharma Company, Resident of Biomedical Technologies Cluster, Skolkovo Foundation, Skolkovo, Moscow Region, Russia

    S. F. Popilyuk

  3. Laboratory No. 16 for Preparatory Biochemistry of Antigens, State Research Center Institute of Immunology, Federal Medial-Biological Agency, Volokolamsk, Russia

    V. L. L’vov

  4. Department of Clinical Immunology and Allergology, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow, Russia

    O. V. Kalyuzhin

Authors
  1. M. V. Kiselevskii
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  2. N. Yu. Anisimova
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  3. S. M. Sitdikova
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  4. F. V. Donenko
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  5. S. F. Popilyuk
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  6. V. L. L’vov
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  7. O. V. Kalyuzhin
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Corresponding author

Correspondence to M. V. Kiselevskii.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 3, pp. 346-350, March, 2019

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Kiselevskii, M.V., Anisimova, N.Y., Sitdikova, S.M. et al. A Combination of Muramylpeptides from Gram-Negative Bacteria Corrects Hematological and Immunological Disorders Induced by Cyclophosphamide. Bull Exp Biol Med 167, 371–374 (2019). https://doi.org/10.1007/s10517-019-04529-6

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  • DOI: https://doi.org/10.1007/s10517-019-04529-6

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