• Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:

Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin

We compared phagocytic activity of macrophages of monocyte origin and Kupffer cells under the influence of M1 and M2 inducers and without activation. Cultures of monocyte-derived macrophages and Kupffer cells were characterized by intensive expression of CD68 that was not affected by activation factors. At the same time, these cultures demonstrated different dynamics of phagocytic activity. Monocyte-derived macrophages initially had more pronounced absorption capacity that gradually increased during the experiment. Kupffer cells were characterized by abrupt fluctuations of phagocytic activity: sharp growth and rapid saturation. Despite these differences, the endosomes produced by monocyte-derived macrophages and Kupffer cells had similar degrees of maturity.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Beattie L, Sawtell A, Mann J, Frame TCM, Teal B, de Labastida Rivera F, Brown N, Walwyn-Brown K, Moore JWJ, MacDonald S, Lim EK, Dalton JE, Engwerda CR, MacDonald KP, Kaye PM. Bone marrow-derived and resident liver macrophages display unique transcriptomic signatures but similar biological functions. J. Hepatol. 2016;65(4):758-768.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Bilzer M, Roggel F, Gerbes AL. Role of Kupffer cells in host defense and liver disease Role of Kupffer cells in host defense and liver disease. Liver Int. 2006;26(10):1175-1186.

    Article  CAS  PubMed  Google Scholar 

  3. 3.

    Chazaud B. Macrophages: supportive cells for tissue repair and regeneration. Immunobiology. 2014;219(3):172-178.

    Article  CAS  PubMed  Google Scholar 

  4. 4.

    Elchaninov AV, Fatkhudinov TK, Usman NY, Kananykhina EY, Arutyunyan IV, Makarov AV, Lokhonina AV, Eremina IZ, Surovtsev VV, Goldshtein DV, Bolshakova GB, Glinkina VV, Sukhikh GT. Dynamics of macrophage populations of the liver after subtotal hepatectomy in rats. BMC Immunol. 2018;19(1). ID 23. doi: https://doi.org/10.1186/s12865-018-0260-1.

  5. 5.

    Gomez Perdiguero E, Klapproth K, Schulz C, Busch K, Azzoni E, Crozet L, Garner H, Trouillet C, de Bruijn MF, Geissmann F, Rodewald HR. Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors. Nature. 2015;518:547-551.

    Article  CAS  PubMed  Google Scholar 

  6. 6.

    Holness CL, da Silva RP, Fawcett J, Gordon S, Simmons DL. Macrosialin, a mouse macrophage-restricted glycoprotein, is a memberof the lamp/lgp family. J. Biol. Chem. 1993; 268(13):9661-9666.

    CAS  PubMed  Google Scholar 

  7. 7.

    Holness CL, Simmons DL. Molecular cloning of CD68, a humanmacrophage marker related to lysosomal glycoproteins. Blood. 1993;81(6):1607-1613.

    CAS  PubMed  Google Scholar 

  8. 8.

    Li P, He K, Li J, Liu Z, Gong J. The role of Kupffer cells in hepatic diseases. Mol. Immunol. 2017;85:222-229.

    Article  CAS  PubMed  Google Scholar 

  9. 9.

    Michalopoulos GK. Advances in liver regeneration. Expert Rev. Gastroenterol. Hepatol. 2014;8(8):897-907.

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Perdiguero EG, Geissmann F. The development and maintenance of resident macrophages. Nat. Immunol. 2016;17(1):2-8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Saito N, Pulford KA, Breton-Gorius J, Massé JM, Mason DY, Cramer EM. Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes. Am. J. Pathol. 1991;139(5):1053-1059.

    CAS  PubMed  PubMed Central  Google Scholar 

  12. 12.

    van Furth R, Cohn ZA, Hirsch JG, Humphrey JH, Spector WG, Langevoort HL. The mononuclear phagocyte system: a new classification of macrophages, monocytes, and their precursor cells. Bull. World Health Organ. 1972;46(6):845-852.

    PubMed  PubMed Central  Google Scholar 

  13. 13.

    You Q, Holt M, Yin H, Li G, Hu CJ, Ju C. Role of hepatic resident and infiltrating macrophages in liver repair after acute injury. Biochem. Pharmacol. 2013;86(6):836-843.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. V. Elchaninov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 37-41, January, 2019

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Lokhonina, A.V., Makarov, A.V., Elchaninov, A.V. et al. Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin. Bull Exp Biol Med 167, 154–158 (2019). https://doi.org/10.1007/s10517-019-04481-5

Download citation

Key Words

  • macrophages
  • Kupffer cells
  • monocytes
  • phagocytosis