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Co-Transplantation of Pancreatic Islet Cells and Mesenchymal Bone Marrow Precursors on Titanium Nickelide Scaffolds in Alloxan-Induced Diabetes Mellitus

  • O. V. KokorevEmail author
  • V. N. Khodorenko
  • V. Yu. Serebrov
  • G. Ts. Dambaev
  • V. E. Gunter
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • 14 Downloads

We studied therapeutic activity of co-transplantation of allogeneic pancreatic islet cells and mesenchymal bone marrow progenitors on TiNi scaffolds in Wistar rats with experimental alloxan-induced diabetes mellitus. In preliminary experiments with co-culturing of cells in different proportions followed by their transplantation on tissue-engineered constructs, the optimum ratio of these cells was determined — 3:1. Regeneration was assessed by biochemical methods by the blood levels of glucose and glycosylated hemoglobin on days 15, 30, and 5. In the group with combined cell transplantation on TiNi scaffold, normalization of the studied biochemical parameters occurred earlier than after monotherapy with allogenic islet cells and was associated with an increase in animal lifespan. Normalization of the parameters of bone marrow hemopoiesis, in particular, the number of myelokaryocytes and erythroblasts was also noted.

Key Words

tissue engineering diabetes mellitus pancreatic islet cells mesenchymal stem cells porous TiNi 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • O. V. Kokorev
    • 1
    • 2
    Email author
  • V. N. Khodorenko
    • 1
  • V. Yu. Serebrov
    • 2
  • G. Ts. Dambaev
    • 2
  • V. E. Gunter
    • 1
  1. 1.Research Institute of Medical Materials and Shape Memory ImplantsNational Research Tomsk State UniversityTomskRussia
  2. 2.Siberian State Medical University, Ministry of Health of the Russian FederationTomskRussia

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