Effect of Interleukin-10 on Localization of AMPA Receptors in Synapses during Long-Term Posttetanic Potentiation in Cultured Hippocampal Slices

  • I. B. MikheevaEmail author
  • A. E. Malkov
  • L. L. Pavlik
  • V. I. Arkhipov
  • S. G. Levin

The effect of the anti-inflammatory cytokine IL-10 on the ultrastructural distribution of AMPA receptor GluR1 subunit in CA1 field of cultured hippocampal slices was studied by using immunohistochemical technique. It was found that long-term posttetanic potentiation increased the content of GluR1 in the postsynaptic density of the axo-spinous synapse. Addition of IL-10 in concentrations of 1 and 10 ng/ml to the medium facilitated long-term posttetanic potentiation thereby changing the distribution of GluR1 in the spine: the number of receptors increased in the cytoplasm and decreased in the postsynaptic density. It is assumed that activation of neuronal IL-10 receptors affects the distribution of AMPA receptors in axo-spinous synapses of hippocampal field CA1 through interplay of intracellular signaling pathways, thereby participating in the mechanisms of synaptic plasticity under normal conditions.

Key Words

interleukin-10 posttetanic potentiation immunocytochemistry GluR1 subunit of AMPA receptors 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Levin SG, Godukhin OV. Modulating effect of cytokines on mechanisms of synaptic plasticity in the brain. Biochemistry (Moscow). 2017;82(3):264-274.CrossRefGoogle Scholar
  2. 2.
    Pavlik LL, Gritsenko EN, Moshkov DA, Mikheeva IB, Talanov EY, Mironova GD. Localization in the cell of the protein forming ATP-dependent potassium-selective channels in a bilayer lipid membrane. An ultrastructural study. Biophysics. 2010;55(5):723-726.CrossRefGoogle Scholar
  3. 3.
    Benke TA, Lüthi A, Isaac JT, Collingridge GL. Modulation of AMPA receptors unitary conductance by synaptic activity. Nature. 1998;393:793-797.CrossRefGoogle Scholar
  4. 4.
    Das P, Lilly SM, Zerda R, Gunning WT 3rd, Alvarez FJ, Tietz EI. Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal. J. Comp. Neurol. 2008;511(6):832-846.CrossRefGoogle Scholar
  5. 5.
    Isaac JT, Nicoll RA, Malenka RC. Evidence for silent synapses: implications for the expression of LTP. Neuron. 1995; 15(2):427-434.CrossRefGoogle Scholar
  6. 6.
    Lobo-Silva D, Carriche GM, Castro AG, Roque S, Saraiva M. Balancing the immune response in the brain: IL-10 and its regulation. J. Neuroinflammation. 2016;13(1):297.CrossRefGoogle Scholar
  7. 7.
    Malinow R, Malenka RC. AMPA receptor trafficking and synaptic plasticity. Annu. Rev. Neurosci. 2002;25:103-126.CrossRefGoogle Scholar
  8. 8.
    Moga DE, Shapiro ML, Morrison JH. Bidirectional redistribution of AMPA but not NMDA receptors after performant path stimulation in the adult hippocampus in vivo. Hippocampus. 2006;16(11):990-1003.CrossRefGoogle Scholar
  9. 9.
    Mosser DM, Zhang X. Interleukin-10: new perspectives on an old cytokine. Immunol. Rev. 2008;226:205-218.CrossRefGoogle Scholar
  10. 10.
    Petralia RS, Wang YX, Wenthold RJ. Internalization at glutamagic synapses during development. Eur. J. Nerosci. 2003; 18(12):3207-3217.CrossRefGoogle Scholar
  11. 11.
    Pinheiro PS, Rodrigues RJ, Silva AP, Cunha RA, Oliveira CR, Malva JO. Solubilization and immunological identification of presynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors in the rat hippocampus. Neurosci. Lett. 2003;336(2):97-100.CrossRefGoogle Scholar
  12. 12.
    Rady PL, Smith EM, Cadet P, Opp MR, Tyring SK, Hughes TK Jr. Presence of interleukin-10 transcripts in human pituitary and hypothalamus. Cell. Mol. Neurobiol. 1995;15(2):289-296.CrossRefGoogle Scholar
  13. 13.
    Roque S, Correia-Neves M, Mesquita AR, Palha JA, Sousa N. Interleukin-10: a key cytokine in depression? Cardiovasc. Psychiatry Neurol. 2009;2009. ID 187894. doi:

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • I. B. Mikheeva
    • 1
    Email author
  • A. E. Malkov
    • 1
  • L. L. Pavlik
    • 1
  • V. I. Arkhipov
    • 1
    • 2
  • S. G. Levin
    • 1
    • 2
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchinoRussia
  2. 2.Pushchino State Institute of Natural SciencesPushchinoRussia

Personalised recommendations