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Selective Action of N-Arachidonoyl Dopamine on Viability and Proliferation of Stromal Cells from Eutopic and Ectopic Endometrium

We performed a comparative study of the cytotoxic effect of endocannabinoid N-arachidonoyl dopamine (AA-DA) on cultured stromal cells of ectopic and eutopic endometrium. It was found that AA-DA in the concentration range of 1-20 μM produces more selective cytotoxic effect on the stromal cells of the ectopic endometrium due to interaction with cannabinoid type 1 receptor. In concentrations below 1 μM, AA-DA stimulated the proliferation of stromal cells of the eutopic endometrium and did not affect the division of ectopic endometrium cells. This effect was realized due to its interaction with cannabinoid type 2 receptor.

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References

  1. Endometriosis: Diagnosis, Treatment and Rehabilitation. Federal Clinical Recommendations. Moscow, 2013. Russian.

  2. Ashba AM, Akimov MG, Gretskaya NM, Bezuglov VV. N-acyl dopamines induce cell death in PC12 cell line via induction of nitric oxide generation and oxidative stress. Doklady Biochem. Biophysics. 2016;467(1):81-84.

    Article  CAS  Google Scholar 

  3. Bobrov MY, Lyzhin AA, Andrianova EL, Gretskaya NM, Zinchenko GN, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-docosahexaenoyl dopamine. Bull. Exp. Biol. Med. 2006;142(4):425-427.

    Article  CAS  PubMed  Google Scholar 

  4. Chernukha GE, Ilyina LM, Adamyan LV, Pavlovich SV. Deep infiltrating endometriosis: Postoperative recurrences and possible ways of their prevention. Akush. Gin. 2015;(8):39-46. Russian.

  5. Akimov MG, Ashba AM, Gretskaya NM, Bezuglov VV. Some aspects of the mechanism of cell death induction by the lipids of N-acyl dopamine family in the PC12 cancer cell line. Anticancer Res. 2014;34(10):5797-5800.

    Google Scholar 

  6. Akimov MG, Gretskaya NM, Zinchenko GN, Bezuglov VV. Cytotoxicity of Endogenous Lipids N-acyl Dopamines and their Possible Metabolic Derivatives for human cancer cell lines of different histological origin. Anticancer Res. 2015;35(5):2657-2661.

    CAS  PubMed  Google Scholar 

  7. Andrianova EL, Bobrov MY, Gretskaya NM, Zinchenko GN, Fomina-Ageeva EV, Bezuglov VV, Serkov IV. The effects of neurolipins and their synthetic analogues on normal and transformed glial cells. Neurochem. J. 2010;4(1):46-54.

    Article  Google Scholar 

  8. Berkley KJ, Rapkin AJ, Papka RE. The pains of endometriosis. Science. 2005;308:1587-1589.

    Article  CAS  PubMed  Google Scholar 

  9. Bobrov MY, Lizhin AA, Andrianova EL, Gretskaya NM, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-arachidonoyldopamine. Neurosci. Lett. 2008;431(1):6-11.

    Article  CAS  PubMed  Google Scholar 

  10. Giudice LC, Kao LC. Endometriosis. Lancet. 2004;364:1789-1799.

    Article  PubMed  Google Scholar 

  11. Liu X, Yamada N, Maruyama W, Osawa T. Formation of dopamine adducts derived from brain polyunsaturated fatty acids mechanism for Parkinson disease. J. Biol. Chem. 2008; 283(50):34,887-34,895.

    Article  CAS  Google Scholar 

  12. Schwarz R, Ramer R, Hinz B. Targeting the endocannabinoid system as a potential anticancer approach. Drug Metab. Rev. 2018;50(1):26-53.

    Article  CAS  PubMed  Google Scholar 

  13. Sanchez AM, Cioffi R, Viganò P, Candiani M, Verde R, Piscitelli F, Di Marzo V, Garavaglia E, Panina-Bordignon P. Elevated systemic levels of endocannabinoids and related mediators across the menstrual cycle in women with endometriosis. Reprod. Sci. 2016;23(8):1071-1079.

    Article  CAS  PubMed  Google Scholar 

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Correspondence to M. Yu. Bobrov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 1, pp. 46-50, January, 2019

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Ashba, A.M., Yushina, M.N., Fedorova-Gogolina, I.A. et al. Selective Action of N-Arachidonoyl Dopamine on Viability and Proliferation of Stromal Cells from Eutopic and Ectopic Endometrium. Bull Exp Biol Med 167, 43–46 (2019). https://doi.org/10.1007/s10517-019-04456-6

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  • DOI: https://doi.org/10.1007/s10517-019-04456-6

Key Words

  • endometriosis
  • stromal cells
  • N-arachidonoyl dopamine
  • cytotoxicity
  • proliferation