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Detection of Low-Abundant MicroRNAs with Hybridization Microchips

  • EXPERIMENTAL BIOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips.

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Authors and Affiliations

  1. P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia

    M. Yu. Shkurnikov, K. A. Fomicheva & Yu. A. Makarova

  2. BioClinicum Company, Moscow, Russia

    E. N. Knyazev

  3. Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow, Russia

    M. S. Kasatkin

  4. Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine, Moscow, Russia

    V. V. Arkov

Authors
  1. M. Yu. Shkurnikov
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  2. E. N. Knyazev
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  3. K. A. Fomicheva
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  4. M. S. Kasatkin
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  5. V. V. Arkov
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  6. Yu. A. Makarova
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Correspondence to M. Yu. Shkurnikov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 12, pp. 757-761, December, 2018

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Shkurnikov, M.Y., Knyazev, E.N., Fomicheva, K.A. et al. Detection of Low-Abundant MicroRNAs with Hybridization Microchips. Bull Exp Biol Med 166, 788–792 (2019). https://doi.org/10.1007/s10517-019-04441-z

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  • DOI: https://doi.org/10.1007/s10517-019-04441-z

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