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Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells

  • Cell Technologies in Biology and Medicine
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit+ cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis. Using proteomic analysis, we identified transgenic SCF in extracellular vesicles released by AAV-modified mesenchymal stromal cells and detected some proteins specific of extracellular vesicles secreted by transduced cells. Extracellular vesicles from AAV-transduced mesenchymal stromal cells could be used for delivery of transgenic proteins as they were readily endocytosed by both cardiosphere-derived cells and cardiac-progenitor cells.

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Authors and Affiliations

  1. National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia

    E. S. Zubkova, I. B. Beloglazova, E. G. Evtushenko, E. K. Shevchenko, K. V. Dergilev, E. I. Ratner, E. V. Parfenova & M. Yu. Men’shikov

  2. M. V. Lomonosov Moscow State University, Moscow, Russia

    E. S. Zubkova, I. B. Beloglazova, K. V. Dergilev & E. V. Parfenova

Authors
  1. E. S. Zubkova
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  2. I. B. Beloglazova
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  3. E. G. Evtushenko
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  4. A. T. Kopylov
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  5. E. K. Shevchenko
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  6. K. V. Dergilev
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  7. E. I. Ratner
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  8. E. V. Parfenova
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  9. M. Yu. Men’shikov
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Corresponding author

Correspondence to E. S. Zubkova.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 211-219, December, 2018

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Zubkova, E.S., Beloglazova, I.B., Evtushenko, E.G. et al. Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells. Bull Exp Biol Med 166, 527–534 (2019). https://doi.org/10.1007/s10517-019-04387-2

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  • DOI: https://doi.org/10.1007/s10517-019-04387-2

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