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Role of Muscarinic M1, M2, and M3 Receptors in the Regulation of Electrical Activity of Myocardial Tissue of Caval Veins during the Early Postnatal Ontogeny

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We studied the influence of blockers of muscarinic M1, M2, and M3 receptors on the effect of acetylcholine in the myocardial tissue of caval veins in rats at the early stage of ontogeny. The experiments were performed on isolated preparations of the right superior vena cava working under their own rhythm. Action potentials were recorded using the standard microelectrode technique. Acetylcholine (1 μM) suppressed automatic activity in the superior vena cava myocardium. Preliminary perfusion of the preparation with non-selective blocker atropine (1 μM) completely abolished the effect of acetylcholine, treatment with M2 receptor blocker AQ-RA 741 (1 μM) led to partial suppression of the effect of acetylcholine. Blockers of M1 and M3 receptors pirenzepine (1 μM) and 4DAMP (0.1 μM) did not suppress the effect of acetylcholine. Thus, the effect of acetylcholine is predominantly realized via M2 receptors, but M3 receptors can also partially mediate its effect in the superior vena cava myocardium in rats at the early stages of ontogeny.

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References

  1. Zefirov TL, Ziyatdinova NI, Saifutdinova LR, Zefirov AL. Cardiac activity and blood pressure in rats during selective blockade of various subtypes of muscarinic cholinoceptors. Bull. Exp. Biol. Med. 2006;141(6):662-665.

    Article  CAS  PubMed  Google Scholar 

  2. Ziyatdinova NI, Sergeeva AM, Dementieva RE, Zefirov TL. Peculiar effects of muscarinic M1, M2, and M3 receptor blockers on cardiac chronotropic function in neonatal rats. Bull. Exp. Biol. Med. 2012;154(1):1-2.

    Article  CAS  PubMed  Google Scholar 

  3. Kuz’min VS, Rozenshtraukh LV. Changes of excitability of the myocardium of the pulmonary veins in rats during adrenergic stimulation. Dokl. Akad. Nauk. 2012;443(4):516. Russian.

    Google Scholar 

  4. Tapilina SV, Abramochkin DV. Decrease in the sensitivity of myocardium to M3 muscarinic receptor stimulation during postnatal ontogenisis. Acta Naturae. 2016;8(2):127-131.

    Article  CAS  Google Scholar 

  5. Abramochkin DV, Tapilina SV, Sukhova GS, Nikolsky EE, Nurullin LF. Functional M3 cholinoreceptors are present in pacemaker and working myocardium of murine heart. Pflugers Arch. 2012;463(4):523-529.

    Article  CAS  PubMed  Google Scholar 

  6. Christoffels VM, Mommersteeg MT, Trowe MO, Prall OW, De Gier-de Vries C, Soufan AT, Bussen M, Schuster-Gossler K, Harvey RP, Moorman AF, Kispert A. Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18. Circ. Res. 2006;98(12):1555-1563.

    Article  CAS  PubMed  Google Scholar 

  7. Dhein S, van Koppen CJ, Brodde OE. Muscarinic receptors in the mammalian heart. Pharmacol. Res. 2001;44(3):161-182.

    Article  CAS  PubMed  Google Scholar 

  8. Haïssaguerre M, Jaïs P, Shah DC, Takahashi A, Hocini M, Quiniou G, Garrigue S, Le Mouroux A, Le Métayer P, Clémenty J. Spontaneous initiation of atrial fibrillation by ectopic beats originating in the pulmonary veins. N. Engl. J. Med. 1998;339(10):659-666.

    Article  PubMed  Google Scholar 

  9. Hardouin SN, Richmond KN, Zimmerman A, Hamilton SE, Feigl EO, Nathanson NM. Altered cardiovascular responses in mice lacking the M(1) muscarinic acetylcholine receptor. J. Pharmacol. Exp. Ther. 2002;301(1):129-137.

    Article  CAS  PubMed  Google Scholar 

  10. Lin WS, Tai CT, Hsieh MH, Tsai CF, Lin YK, Tsao HM, Huang JL, Yu WC, Yang SP, Ding YA, Chang MS, Chen SA. Catheter ablation of paroxysmal atrial fibrillation initiated by non-pulmonary vein ectopy. Circulation. 2003;107(25):3176-3183.

    Article  PubMed  Google Scholar 

  11. Mommersteeg MT, Christoffels VM, Anderson RH, Moorman AF. Atrial fibrillation: a developmental point of view. Heart Rhythm. 2009;6(12):1818-1824.

    Article  PubMed  Google Scholar 

  12. Shi H, Wang H, Yang B, Xu D, Wang Z. The M3 receptormediated K(+) current (IKM3), a G(q) protein-coupled K(+) channel. J. Biol. Chem. 2004;279(21):21,774-21,778.

    Article  CAS  Google Scholar 

  13. Tapilina SV, Abramochkin DV. Different myocardial sensitivity in newborn and mature rats to selective stimulation of M3 cholinoreceptors. Bull. Exp. Biol. Med. 2015;159(1):8-10.

    Article  CAS  PubMed  Google Scholar 

  14. Tsai CF, Tai CT, Hsieh MH, Lin WS, Yu WC, Ueng KC, Ding YA, Chang MS, Chen SA. Initiation of atrial fibrillation by ectopic beats originating from the superior vena cava: electrophysiological characteristics and results of radiofrequency ablation. Circulation. 2000;102(1):67-74.

    Article  CAS  PubMed  Google Scholar 

  15. Woo SH, Lee BH, Kwon KI, Lee CO. Excitatory effect of M1 muscarinic acetylcholine receptor on automaticity of mouse heart. Arch. Pharm. Res. 2005;28(8):930-935.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Human and Animal Physiology, M. V. Lomonosov Moscow State University, Ministry of Health of the Russian Federation, Moscow, Russia

    A. D. Ivanova, S. V. Tapilina & V. S. Kuz’min

  2. Department of Physiology, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    S. V. Tapilina & V. S. Kuz’min

Authors
  1. A. D. Ivanova
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  2. S. V. Tapilina
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  3. V. S. Kuz’min
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Correspondence to A. D. Ivanova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 10, pp. 404-409, October, 2018

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Ivanova, A.D., Tapilina, S.V. & Kuz’min, V.S. Role of Muscarinic M1, M2, and M3 Receptors in the Regulation of Electrical Activity of Myocardial Tissue of Caval Veins during the Early Postnatal Ontogeny. Bull Exp Biol Med 166, 421–425 (2019). https://doi.org/10.1007/s10517-019-04364-9

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  • DOI: https://doi.org/10.1007/s10517-019-04364-9

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