Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 3, pp 409–412 | Cite as

Analysis of the Intensity of Apoptosis in Rat Organs at Various Stages of Ontogeny

  • N. V. TyshkoEmail author
  • E. O. Sadykova
  • S. I. Shestakova
  • E. N. Trushina
  • O. K. Mustafina

Activity of apoptosis in the thymus, liver, and kidneys on days 20, 22, 35, 50, 80, 110, and 140 of ontogeny was studied in the experiments on rats using the alkaline gel electrophoresis and flow cytofluorometry. Changes in apoptosis intensity depended on animal age. The maximum level of this parameter was observed on day 20 of ontogeny with the following reduction in this parameter to the minimum value on days 35-50. Then it gradually increased up to relatively high levels on day 110 and significantly reduced by day 140.

Key Words

apoptosis ontogeny biomarkers toxicological studies 


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  1. 1.
    Durnev AD, Zhanataev AK, Anisina EA, Sidneva ES, Nikitina VA, Oganesyants LA, Seredin SB, Bekish VYa, Chernukha IM. Application of Alkaline Gel Electrophoresis of Isolated Cells for Assessment of Genotoxicity of Natural and Synthetic Compounds. Methodical Recommendation. Moscow, 2006. Vol. 2. Russian.Google Scholar
  2. 2.
    Methodical instructions MU Biomedical assessment of the safety of genetically modified organisms of plant origin. Supervision over the production and circulation of food products containing GMO: Guidelines. Moscow, 2008. Russian.Google Scholar
  3. 3.
    Tishko NV, Zhminchenko VM, Pashorina VA, Selyaskin KE, Mel’nik EA, Mustafina OK, Soto SKh, Trushina EN, Gapparov MM. A comparative assessment of the diet influence on growth and development of rats. Vopr. Pitaniya. 2011;80(5):30. Russian.Google Scholar
  4. 4.
    Tyshko NV, Selyaskin KE, Tutelyan VA. Detection of rat internal organs apoptosis activity based on model of CCl4 toxic effects. Fundament. Issked. 2014;(10-5):993-998. Russian.Google Scholar
  5. 5.
    Derelanko MJ. Handbook of Toxicology. Boca Raton, 2001.Google Scholar
  6. 6.
    Developmental and Reproductive Toxicology: a Practical Approach. Hood RD, ed. Boca Raton, 2006.Google Scholar
  7. 7.
    Genetically Modified Food Sources. Safety Assessment and Control. Tutelyan VA, ed. New York, 2013.Google Scholar
  8. 8.
    John CR, Douglas RG. Apoptosis: Physiology and Pathology. Cambridge, 2011.Google Scholar
  9. 9.
    Ouyang L, Shi Z, Zhao S, Wang FT, Zhou TT, Liu B, Bao JK. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif. 2012;45(6):487-498.CrossRefGoogle Scholar
  10. 10.
    Smith CC, Adkins DJ, Martin EA, O’Donovan MR. Recommendations for design of the rat comet assay. Mutagenesis. 2008;23(3):233-240.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • N. V. Tyshko
    • 1
    Email author
  • E. O. Sadykova
    • 1
  • S. I. Shestakova
    • 1
  • E. N. Trushina
    • 1
  • O. K. Mustafina
    • 1
  1. 1.Federal Research Center of Nutrition and BiotechnologyMoscowRussia

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