Skip to main content
SpringerLink
Log in

Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages

  • BIOTECHNOLOGIES
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bianco A, Kostarelos K, Prato M. Applications of carbon nanotubes in drug delivery. Curr. Opin. Chem. Biol. 2005;9(6):674-679.

    Article  CAS  PubMed  Google Scholar 

  2. Dai X, Tan Y, Xu J. Formation of gold nanoparticles in the presence of o-anisidine and the dependence of the structure of poly(o-anisidine) on synthetic conditions. Langmuir. 2002;18(23):9010-9016.

    Article  CAS  Google Scholar 

  3. Das M, Shim KH, An SSA, Yi DK. Review on gold nanoparticles and their applications. Toxicol. Environ. Health Sci. 2011;3(4):193-205.

    Article  Google Scholar 

  4. Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br. J. Pharmacol. 2004;142(2):231-255.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Hao R, Xing R, Xu Z, Hou Y, Gao S, Sun S. Synthesis, functionalization, and biomedical applications of multifunctional magnetic nanoparticles. Adv. Mater. 2010;22(25):2729-2742.

    Article  CAS  PubMed  Google Scholar 

  6. Haume K, Rosa S, Grellet S, Śmiałek MA, Butterworth KT, Solov’yov AV, Prise KM, Golding J, Mason NJ. Gold nanoparticles for cancer radiotherapy: a review. Cancer Nanotechnol. 2016;7(1):8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Hornos Carneiro MF, Barbosa F Jr. Gold nanoparticles: A critical review of therapeutic applications and toxicological aspects. J. Toxicol. Environ. Health B Crit. Rev. 2016;19(3-4):129-148.

    Article  CAS  PubMed  Google Scholar 

  8. Kam NW, O’Connell M, Wisdom JA, Dai H. Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. Proc. Natl Acad. Sci. USA. 2005;102(33):11,600-11,605.

    Article  CAS  Google Scholar 

  9. Lunov O, Syrovets T, Büchele B, Jiang X, Röcker C, Tron K, Nienhaus GU, Walther P, Mailänder V, Landfester K, Simmet T. The effect of carboxydextran-coated superparamagnetic iron oxide nanoparticles on c-Jun N-terminal kinase-mediated apoptosis in human macrophages. Biomaterials. 2010;31(19): 5063-5071.

    Article  CAS  PubMed  Google Scholar 

  10. Machulkin AE, Garanina AS, Zhironkina OA, Beloglazkina EK, Zyk NV, Savchenko AG, Kotelyanskii VE, Mazhuga AG. Nanohybride materials based on magnetite-gold nanoparticles for diagnostics of prostate cancer: synthesis and in vitro testing. Bull. Exp. Biol. Med. 2016;161(5):706-710.

    Article  CAS  PubMed  Google Scholar 

  11. Qiao R, Yang C, Gao M. Superparamagnetic iron oxide nanoparticles: from preparations to in vivo MRI applications. J. Mater. Chem. 2009;19(35):6274-6293.

    Article  CAS  Google Scholar 

  12. Pirutin SK, Turovetsky VB, Sarycheva NY, Kamensky AA, Druzhko AB, Kalihevich VN. Influence of tetrapeptide tuftsin on intracellular ph of mouse peritoneal macrophages. Moscow University Biol. Sci. Bull. 2016;71(1):58-61.

    Article  Google Scholar 

  13. Tse BW, Cowin GJ, Soekmadji C, Jovanovic L, Vasireddy RS, Ling MT, Khatri A, Liu T, Thierry B, Russell PJ. PSMAtargeting iron oxide magnetic nanoparticles enhance MRI of preclinical prostate cancer. Nanomedicine. 2014;10(3):375-386.

    Article  CAS  PubMed  Google Scholar 

  14. Wang YX. Superparamagnetic iron oxide based MRI contrast agents: Current status of clinical application. Quant. Imaging Med. Sur. 2011;1(1):36-40.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. M. V. Lomonosov Moscow State University, Moscow, Russia

    S. K. Pirutin, M. V. Efremova, A. I. Yusipovich, V. B. Turovetskii, G. V. Maksimov & A. G. Mazhuga

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

    S. K. Pirutin & A. B. Druzhko

  3. National University of Science and Technology MISIS, Moscow, Russia

    M. V. Efremova & A. G. Mazhuga

  4. D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

    A. G. Mazhuga

Authors
  1. S. K. Pirutin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Efremova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. I. Yusipovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. B. Turovetskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. V. Maksimov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. B. Druzhko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. G. Mazhuga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. K. Pirutin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 9, pp. 362-365, September, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pirutin, S.K., Efremova, M.V., Yusipovich, A.I. et al. Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages. Bull Exp Biol Med 166, 386–389 (2019). https://doi.org/10.1007/s10517-019-04356-9

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-019-04356-9

Key Words

Search

Navigation