We propose an in vitro model of chronic oxidative stress based on the use of glucose oxidase. Oxidative stress modeling leads to a significant increase in the number of dead cells in culture. It was shown that the glial cell-derived neurotrophic factor exhibits a pronounced anti-oxidant effect. Preventive application of 1 ng/ml glial cell-derived neurotrophic factor significantly reduced the percentage of dead cells in culture.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 8, pp. 257-260, August, 2018
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Mishchenko, T.A., Mitroshina, E.V., Shishkina, T.V. et al. Antioxidant Properties of Glial Cell-Derived Neurotrophic Factor (GDNF). Bull Exp Biol Med 166, 293–296 (2018). https://doi.org/10.1007/s10517-018-4335-z
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DOI: https://doi.org/10.1007/s10517-018-4335-z