Based on the data of morphological analysis, we performed histological evaluation of rat tissue reaction to subcutaneous implantation of decellularized matrices of intrathoracic organs and tissues. Cell composition of the inflammatory infiltrate was analyzed, and the dynamics of macrophage and T and B lymphocyte content was assessed on days 7 and 14 of the experiment. It was found that the reaction to implantation depended not only on the quality of decellularization and efficiency of removal of antigen molecules, but also on the original histological structure and quality of preimplantation processing of the transplant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kuevda EV, Gubareva EA, Sotnichenko AS, Gumenyuk IS, Gilevich IV, Polyakov IS, Porkhanov VA, Makkiarini PR. Detergent-enzymatic method of rat lung decellularization. Matrix morphological evaluation. Sovremen. Problemy Nauki Obrazovaniya. 2015;(3):44. Russian.
Sotnichenko AS, Gubareva EA, Kuevda EV, Gumenyuk IS, Gilevich IV, Nakokhov RZ, Slavinskiy AA, Alekseenko SN. Modern outlook on morphological criteria of organ and tissue decellularization. Vestn. Transplantol. Iskysstv. Organov. 2017;19(3):65-69. Russian.
Sotnichenko AS, Slavinskiy AA, Gilevich IV, Kuevda EV, Gumenyuk IS, Gubareva EA, Makkiarini PR. Pathomorphological characterizations of tissue-engineered rat heart scaffold. Sovremen. Problemy Nauki Obrazovaniya. 2015;(2):44. Russian.
Shekhter AB, Guller AE, Istranov LP, Istranova EV, Butnaru DV, Vinarov AZ, Zakharkina OL, Kurkov AV, Kantimerov DF, Antonov EN, Marisov LV, Glybochko PV. Morphology of collagen matrices for tissue engineering (biocompatibility, biodegradation, tissue response). Arkh. Patol. 2015;77(6):29-38. Russian.
Gilbert TW. Strategies for tissue and organ decellularization. J. Cell. Biochem. 2012;113(7):2217-2222.
Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006;27(19):3675-3683.
Gubareva EA, Sjöqvist S, Gilevich IV, Sotnichenko AS, Kuevda EV, Lim ML, Feliu N, Lemon G, Danilenko KA, Nakokhov RZ, Gumenyuk IS, Grigoriev TE, Krasheninnikov SV, Pokhotko AG, Basov AA, Dzhimak SS, Gustafsson Y, Bautista G, Beltrán Rodríguez A, Pokrovsky VM, Jungebluth P, Chvalun SN, Holterman MJ, Taylor DA, Macchiarini P. Orthotopic transplantation of a tissue engineered diaphragm in rats. Biomaterials. 2016;77:320-335.
He M, Callanan A. Comparison of methods for whole-organ decellularization in tissue engineering of bioartificial organs. Tissue Eng. Part B Rev. 2012;19(3):194-208.
Keane TJ, Londono R, Turner NJ, Badylak SF. Consequences of ineffective decellularization of biologic scaffolds on the host response. Biomaterials. 2012;33(6):1771-1781.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 8, pp. 251-256, August, 2018
Rights and permissions
About this article
Cite this article
Sotnichenko, A.S., Nakokhov, R.Z., Gubareva, E.A. et al. Morphological Evaluation of the Tissue Reaction to Subcutaneous Implantation of Decellularized Matrices. Bull Exp Biol Med 166, 287–292 (2018). https://doi.org/10.1007/s10517-018-4334-0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4334-0