Skip to main content
SpringerLink
Log in

Hydrogen Peroxide Treatment of Muscle Fibres Inhibits the Formation of Myosin Cross-Bridges

  • PHYSIOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The molecular mechanism of violation of the contractile function of skeletal muscles caused by oxidative damage to myosin is not fully understood. Using permeabilized fibres from fast (m. psoas) and slow (m. soleus) rabbit muscles, we studied the effect of myosin oxidation on the mechanism of force generation and its calcium regulation. It was found that this treatment simultaneously reduces the maximum force and fibers stiffness without affecting their calcium sensitivity. This suggests that the mechanism of oxidation-related impairment the force-generating ability of fibers consists in suppression of myosin cross-bridges formation and does no affect the characteristics of actin—myosin interaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andrade FH, Reid MB, Allen DG, Westerblad H. Effect of hydrogen peroxide and dithiothreitol on contractile function of single skeletal muscle fibres from the mouse. J. Physiol. 1998;509(2):565-575.

    Article  CAS  Google Scholar 

  2. Bershitsky SY, Tsaturyan AK. The elementary force generation process probed by temperature and length perturbations in muscle fibres from the rabbit. J. Physiol. 2002;540(3):971-988.

    Article  CAS  Google Scholar 

  3. Chang CC, Yang MH, Tung HC, Chang CY, Tsai YL, Huang JP, Yen TH, Hung LM. Resveratrol exhibits differential protective effects on fast- and slow-twitch muscles in streptozotocininduced diabetic rats. J. Diabetes. 2014;6(1):60-67.

    Article  CAS  Google Scholar 

  4. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clin. Chim. Acta. 2003;329(1-2):23-38.

    Article  CAS  Google Scholar 

  5. Dutka TL, Verburg E, Larkins N, Hortemo KH, Lunde PK, Sejersted OM, Lamb GD. ROS-mediated decline in maximum Ca2+-activated force in rat skeletal muscle fibers following in vitro and in vivo stimulation. PLoS One. 2012;7(5):e35226. doi: https://doi.org/10.1371/journal.pone.0035226.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Gross SM, Lehman SL. Accessibility of myofilament cysteines and effects on ATPase depend on the activation state during exposure to oxidants. PLoS One. 2013;8(7):e69110. doi: https://doi.org/10.1371/journal.pone.0069110.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Lamb GD, Posterino GS. Effects of oxidation and reduction on contractile function in skeletal muscle fibres of the rat. J. Physiol. 2003;546(1):149-163.

    Article  CAS  Google Scholar 

  8. Lamb GD, Westerblad H. Acute effects of reactive oxygen and nitrogen species on the contractile function of skeletal muscle. J. Physiol. 2011;589(9):2119-2127.

    Article  CAS  Google Scholar 

  9. Murphy RM, Dutka TL, Lamb GD. Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres. J. Physiol. 2008;586(8):2203-2216.

    Article  CAS  Google Scholar 

  10. Plant DR, Lynch GS, Williams DA. Hydrogen peroxide modulates Ca2+-activation of single permeabilized fibres from fastand slow-twitch skeletal muscles of rats. J. Muscle Res. Cell Motil. 2000;21(8):747-752.

    Article  CAS  Google Scholar 

  11. Prochniewicz E, Lowe DA, Spakowicz DJ, Higgins L, O’Conor K, Thompson LV, Ferrington DA, Thomas DD. Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers. Am. J. Physiol. Cell Physiol. 2008;294(2):C613-C626.

    Article  CAS  Google Scholar 

  12. Zergeroglu MA, McKenzie MJ, Shanely RA, Van Gammeren D, DeRuisseau KC, Powers SK. Mechanical ventilationinduced oxidative stress in the diaphragm. J. Appl. Physiol. (1985). 2003;95(3):1116-1124.

    Article  CAS  Google Scholar 

  13. Yamada T, Mishima T, Sakamoto M, Sugiyama M, Matsunaga S, Wada M. Oxidation of myosin heavy chain and reduction in force production in hyperthyroid rat soleus. J. Appl. Physiol. (1985). 2006;100(5):1520-1526.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Yekaterinburg, Russia

    P. V. Kochubei, G. V. Kopylova, D. V. Shchepkin & S. Yu. Bershitskii

Authors
  1. P. V. Kochubei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. V. Kopylova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. V. Shchepkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Yu. Bershitskii
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. V. Kopylova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 8, pp. 136-140, August, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kochubei, P.V., Kopylova, G.V., Shchepkin, D.V. et al. Hydrogen Peroxide Treatment of Muscle Fibres Inhibits the Formation of Myosin Cross-Bridges. Bull Exp Biol Med 166, 183–187 (2018). https://doi.org/10.1007/s10517-018-4310-8

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4310-8

Key Words

Search

Navigation