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Assessment of Lactate Production and Proteoglycans Synthesis by the Intact and Degenerated Intervertebral Disc Cells under the Influence of Activated Macrophages: an In Vitro Study

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The effects of proinflammatory cytokines on the secretion of glycosaminoglycans and lactate production by normal and degenerated intervertebral disk cells were studied on the model of their co-culturing with activated macrophage-like cells. It was found that proinflammatory cytokines produced a direct effect on intervertebral disk cells in a 3D culture reducing the rate of glycolysis and synthetic activity of both normal and degenerated cells of annulus fibrosus and nucleus pulposus, which is an important factor in progression of intervertebral disk degeneration.

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Authors and Affiliations

  1. Irkutsk State Medical University, Irkutsk, Russia

    V. A. Byvaltsev, L. A. Bardonova, E. G Belykh & L. I. Korytov

  2. Irkutsk Research Center of Surgery and Traumatology, Irkutsk, Russia

    V. A. Byvaltsev

  3. Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company, Irkutsk, Russia

    V. A. Byvaltsev

  4. Research Center for Problems of Family Health and Human Reproduction, Irkutsk, Russia

    S. I. Kolesnikov

  5. M. V. Lomonosov Moscow State University, Moscow, Russia

    S. I. Kolesnikov

  6. Oregon State University, Corvallis, OR, USA

    M. B. Giers

  7. Barrow Neurological Institute, Phoenix, AZ, USA

    L. A. Bardonova, E. G Belykh & M. C. Preul

Authors
  1. V. A. Byvaltsev
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  2. S. I. Kolesnikov
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  3. L. A. Bardonova
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  4. E. G Belykh
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  5. L. I. Korytov
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  6. M. B. Giers
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  7. M. C. Preul
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Corresponding author

Correspondence to V. A. Byvaltsev.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 197-200, September, 2018

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Byvaltsev, V.A., Kolesnikov, S.I., Bardonova, L.A. et al. Assessment of Lactate Production and Proteoglycans Synthesis by the Intact and Degenerated Intervertebral Disc Cells under the Influence of Activated Macrophages: an In Vitro Study. Bull Exp Biol Med 166, 170–173 (2018). https://doi.org/10.1007/s10517-018-4307-3

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  • DOI: https://doi.org/10.1007/s10517-018-4307-3

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