Skip to main content
  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:

Assessment of Lactate Production and Proteoglycans Synthesis by the Intact and Degenerated Intervertebral Disc Cells under the Influence of Activated Macrophages: an In Vitro Study

Bulletin of Experimental Biology and Medicine volume 166, pages 170–173 (2018)Cite this article

The effects of proinflammatory cytokines on the secretion of glycosaminoglycans and lactate production by normal and degenerated intervertebral disk cells were studied on the model of their co-culturing with activated macrophage-like cells. It was found that proinflammatory cytokines produced a direct effect on intervertebral disk cells in a 3D culture reducing the rate of glycolysis and synthetic activity of both normal and degenerated cells of annulus fibrosus and nucleus pulposus, which is an important factor in progression of intervertebral disk degeneration.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Rusova TV, Kuleshova ON, Zhukov DV. Glycosaminoglycans of intervertebral disc tissues in patients with idiopathic scoliosis. Khirurg. Pozvonochnika. 2006;(3):84-87. Russian.

  2. Stepanov IA, Bardonova LA, Belykh YG, Byvaltsev VA. Embryogenesis and Regeneration of the Intervertebral Disk (Review). Sovremen. Tekhnol. Med. 2017;9(3):151-161. Russian.

    Article  Google Scholar 

  3. Tabeeva GR. Low back pain: possibilities of pathogenetic treatment. Zh. Nevrol. Psikhiatr. 2015;115(12):162-168. Russian.

    CAS  Article  Google Scholar 

  4. Ab-Rahim S, Selvaratnam L, Raghavendran HR, Kamarul T. Chondrocyte-alginate constructs with or without TGF-b1 produces superior extracellular matrix expression than monolayer cultures. Mol. Cell. Biochem. 2013;376(1-2):11-20.

    CAS  Article  Google Scholar 

  5. Adams MA, Roughley PJ. What is intervertebral disc degeneration, and what causes it? Spine (Phila Pa 1976). 2006;31(18):2151-2161.

    Article  Google Scholar 

  6. Hwang MH, Shin JH, Kim KS, Yoo CM, Jo GE, Kim JH, Choi H. Low level light therapy modulates inflammatory mediators secreted by human annulus fibrosus cells during intervertebral disc degeneration in vitro. Photochem. Photobiol. 2015;91(2):403-410.

    CAS  Article  Google Scholar 

  7. Kim JH, Studer RK, Sowa GA, Vo NV, Kang JD. Activated macrophage-like THP-1 cells modulate anulus fibrosus cell production of inflammatory mediators in response to cytokines. Spine (Phila Pa 1976). 2008;33(21):2253-2259.

    Article  Google Scholar 

  8. Kluba T, Niemeyer T, Gaissmaier C, Gründer T. Human anulus fibrosis and nucleus pulposus cells of the intervertebral disc: Effect of degeneration and culture system on cell phenotype. Spine (Phila Pa 1976). 2005;30(24):2743-2748.

    Article  Google Scholar 

  9. Le Maitre CL, Freemont AJ, Hoyland JA. The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res. Ther. 2005;7(4):R732-R745.

    Article  Google Scholar 

  10. Park JJ, Moon HJ, Park JH, Kwon TH, Park YK, Kim JH. Induction of proinflammatory cytokine production in intervertebral disc cells by macrophage-like THP-1 cells requires mitogen-activated protein kinase activity. J. Neurosurg. Spine. 2016;24(1):167-175.

    Article  Google Scholar 

  11. Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J, Boos N. Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 2001;26(17):1873-1878.

    CAS  Article  Google Scholar 

  12. Risbud MV, Shapiro IM. Role of cytokines in intervertebral disc degeneration: pain and disc content. Nat. Rev. Rheumatol. 2014;10(1):44-56.

    CAS  Article  Google Scholar 

  13. Salvatierra JC, Yuan TY, Fernando H, Castillo A, Gu WY, Cheung HS, Huant CY. Difference in energy metabolism of annulus fibrosus and nucleus pulposus cells of the intervertebral disc. Cell. Mol. Bioeng. 2011;4(2):302-310.

    Article  Google Scholar 

  14. Shamji MF, Setton LA, Jarvis W, So S, Chen J, Jing L, Bullock R, Isaacs RE, Brown C, Richardson WJ. Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum. 2010;62(7):1974-1982.

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Yamamoto J, Maeno K, Takada T, Kakutani K, Yurube T, Zhang Z, Hirata H, Kurakawa T, Sakai D, Mochida J, Doita M, Kurosaka M, Nishida K. Fas ligand plays an important role for the production of pro-inflammatory cytokines in intervertebral disc nucleus pulposus cells. J. Orthop. Res. 2013;31(4):608-615.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

  1. Irkutsk State Medical University, Irkutsk, Russia

    V. A. Byvaltsev, L. A. Bardonova, E. G Belykh & L. I. Korytov

  2. Irkutsk Research Center of Surgery and Traumatology, Irkutsk, Russia

    V. A. Byvaltsev

  3. Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company, Irkutsk, Russia

    V. A. Byvaltsev

  4. Research Center for Problems of Family Health and Human Reproduction, Irkutsk, Russia

    S. I. Kolesnikov

  5. M. V. Lomonosov Moscow State University, Moscow, Russia

    S. I. Kolesnikov

  6. Oregon State University, Corvallis, OR, USA

    M. B. Giers

  7. Barrow Neurological Institute, Phoenix, AZ, USA

    L. A. Bardonova, E. G Belykh & M. C. Preul

Authors
  1. V. A. Byvaltsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. I. Kolesnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. A. Bardonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. G Belykh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. I. Korytov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. B. Giers
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. C. Preul
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. A. Byvaltsev.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 197-200, September, 2018

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Byvaltsev, V.A., Kolesnikov, S.I., Bardonova, L.A. et al. Assessment of Lactate Production and Proteoglycans Synthesis by the Intact and Degenerated Intervertebral Disc Cells under the Influence of Activated Macrophages: an In Vitro Study. Bull Exp Biol Med 166, 170–173 (2018). https://doi.org/10.1007/s10517-018-4307-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4307-3

Key Words

  • intervertebral disc degeneration
  • proinflammatory cytokines
  • lactate
  • proteoglycans