Skip to main content
SpringerLink
Log in

Effect of 30-Day Hindlimb Unloading and Hypergravity on Bone Marrow Stromal Progenitors in C57Bl/6N Mice

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of 30-day hindlimb unloading and subsequent simulated hypergravity on the cellularity and proliferative, clonogenic, and differentiation potential of bone marrow stromal progenitors in mice. Clonogenic and differentiation activity of stromal cells decreased after unloading; proliferative and differentiation activity of bone marrow stromal progenitors increased after hypergravity simulation. Our findings demonstrated negative effect of unloading on functional activity of mouse bone marrow stromal progenitors. Short-term hypergravity after unloading produced a stimulating effect on the bone marrow stromal progenitors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Buravkova LB, Gershovich PM, Gershovich JG, Grigorev AI. Mechanisms of gravitational sensitivity of osteogenic precursor cells. Acta Naturae. 2010;2(1):28-35.

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Vil’-Vil’yams IF. Basic approaches to the choice of shortradius centrifugation regimens in long-term space flights. Aviakosm. Ekol. Med. 1993;5(6):46-51. Russian.

    Google Scholar 

  3. Markina EA, Bobyleva PI, Andrianova IV, Andreeva ER, Buravkova LB. Profile of the marrow-derived stromal precursors population in С57Вl/6N mice flown on biosatellite Bion-M1. Aviakosm. Ekol. Med. 2015;49(6):41-48. Russian.

    CAS  Google Scholar 

  4. Markina EA, Kokhan VS, Roe MP, Andrianova IV, Stemberg AS, Buravkova LB. The influence of radiation and hindlimb unloading on rats’ bone marrow progenitor cells. Tsitologiya. 2017;59(12):846-856. Russian.

    Google Scholar 

  5. Rudimov EG, Buravkov SV, Andreeva EP, Buravkova LB. Effect of proinflammatory activation on F-actin distribution in cultured human endothelial cells under conditions of experimental microgravity. Bull. Exp. Biol. Med. 2015;158(4):573-580.

    Article  CAS  Google Scholar 

  6. Sotnezova EV, Markina EA, Andreeva ER, Buravkova LB. Myeloid Precursors in the Bone Marrow of Mice after a 30-Day Space Mission on a Bion-M1 Biosatellite. Bull. Exp. Biol. Med. 2017;162(4):496-500.

    Article  CAS  Google Scholar 

  7. Shtemberg AS. The problems of experimental investigation of spaceflight factors combined influence on animals organism functions. Ross. Fiziol. Zh. 2014;100(10):1152-1168. Russian.

    CAS  Google Scholar 

  8. Shtemberg AS, Kudrin VS, Klodt PM, Narkevich VB, Bazyan AS. The effects of antiorthostatic hypodynamia and overload on discriminant learning and monoamine exchange in the brain structures of mice. Neurochem. J. 2012;6(4):291-298.

    Article  Google Scholar 

  9. Basso N, Jia Y, Bellows CG, Heersche JN. The effect of reloading on bone volume, osteoblast number, and osteoprogenitor characteristics: studies in hind limb unloaded rats. Bone. 2005;37(3):370-378.

    Article  Google Scholar 

  10. Globus RK, Morey-Holton E. Hindlimb unloading: rodent analog for microgravity. J. Appl. Physiol. 2016;120(10):1196-1206.

    Article  CAS  Google Scholar 

  11. Hamrick MW, Shi X, Zhang W, Pennington C, Thakore H, Haque M, Kang B, Isales CM, Fulzele S, Wenger KH. Loss of myostatin (GDF8) function increases osteogenic differentiation of bone marrow-derived mesenchymal stem cells but the osteogenic effect is ablated with unloading. Bone. 2007;40(6):1544-1553.

    Article  CAS  Google Scholar 

  12. Huang Y, Dai ZQ, Ling SK, Zhang HY, Wan YM, Li YH. Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells. J. Biomed. Sci. 2009;16:87. doi: https://doi.org/10.1186/1423-0127-16-87.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Iwasaki K, Hirayanagi KI, Sasaki T, Kinoue T, Ito M, Miyamoto A, Igarashi M, Yajima K. Effects of repeated long duration +2Gz load on man’s cardiovascular function. Acta Astronaut. 1998;42(1-8):175-183.

    Article  CAS  Google Scholar 

  14. Kawao N, Morita H, Obata K, Tamura Y, Okumoto K, Kaji H. The vestibular system is critical for the changes in muscle and bone induced by hypergravity in mice. Physiol. Rep. 2016;4(19). pii: e12979.

    Article  Google Scholar 

  15. Meirelles Lda S, Nardi NB. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br. J. Haematol. 2003;123(4):702-711.

    Article  Google Scholar 

  16. Shahnazari M, Kurimoto P, Boudignon BM, Orwoll BE, Bikle DD, Halloran BP. Simulated spaceflight produces a rapid and sustained loss of osteoprogenitors and an acute but transitory rise of osteoclast precursors in two genetic strains of mice. Am. J. Physiol. Endocrinol. Metab. 2012;303(11):E1354-E1362.

    Article  CAS  Google Scholar 

  17. Visigalli D, Strangio A, Palmieri D, Manduca P. Hind limb unloading of mice modulates gene expression at the protein and mRNA level in mesenchymal bone cells. BMC Musculoskelet. Disord. 2010;11:147. doi: https://doi.org/10.1186/1471-2474-11-147.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Zhou S, Zu Y, Sun Z, Zhuang F, Yang C. Effects of hypergravity on osteopontin expression in osteoblasts. PLoS One. 2015;10(6):e0128846. doi: https://doi.org/10.1371/journal.pone.0128846.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Research Center Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    E. A. Markina, I. V. Andrianova, A. S. Shtemberg & L. B. Buravkova

Authors
  1. E. A. Markina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. V. Andrianova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. S. Shtemberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. B. Buravkova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. A. Markina.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 152-156, September, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Markina, E.A., Andrianova, I.V., Shtemberg, A.S. et al. Effect of 30-Day Hindlimb Unloading and Hypergravity on Bone Marrow Stromal Progenitors in C57Bl/6N Mice. Bull Exp Biol Med 166, 130–134 (2018). https://doi.org/10.1007/s10517-018-4301-9

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4301-9

Key Words

Search

Navigation