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Extracellular DNA in the Dynamics of Uncomplicated Pregnancy

  • EXPERIMENTAL METHODS FOR CLINIC
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We analyzed the concentration of extracellular DNA and its fractions in the dynamics of uncomplicated pregnancy Thirty women with singleton pregnancy were examined. The concentration of total, maternal, and fetal cell-free DNA in maternal blood was measured at gestation weeks 11-14, 24-26, and 30-32. The level of total cell-free DNA was evaluated by measuring the concentration of RASSF1A gene using quantitative PCR analysis, the level of cell-free fetal DNA was assessed by determining the hypermethylated part of RASSF1A gene. The concentration of total cell-free DNA and cell-free maternal DNA did not change during the first half of pregnancy, but increased after 24-26 weeks. The level of cell-free fetal DNA increased from the first to the second and third trimester: 14.15 (2.32-36.25), 24.87 (6.29-129.32), and 32.62 (8.97-133.52) GE/ml (p<0.05), respectively. Our results characterize the dynamics of the content of cell-free DNA and its fractions during pregnancy, which should be taken into account when using cell-free DNA for prediction of placenta-associated complications.

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References

  1. Karapetyan AO, Krasnyi AM, Sadekova AA, Khlestova GV, Balashov IS, Baev OR. A change in the concentration of extracellular DNA during pregnancy. Akush. Gin. 2018;(3):44-50. Russian.

    Article  Google Scholar 

  2. Krasnyi AM, Kan NE, Tyutyunnik VL, Khovkhaeva PA, Volgina NE, Sergunina OA, Tyutyunnik NV, Bednyagin LA. Oxidative stress in preeclampsia and in normal pregnancy. Akush. Gin. 2016;(5):90-95. Russian.

    Article  Google Scholar 

  3. Sukhikh GT, Trofimov DYu, Barkov IYu, Donnikov AE, Shubina ES, Korostin DO, Ekimov AN, Goltsov AY, Bakharev VA, Karetnikova NA, Borovikov PI, Tetruashvili NK, Kim LV, Gata AS, Pavlovich SV, Skryabin KG, Prokhorchuk EB, Mazur AM, Pantyukh KS. New approaches to prenatal screening for chromosomal abnormalities: Maternal blood DNA screening. Akush. Gin. 2016;(8):72-78. Russian.

    Article  Google Scholar 

  4. Alberry M, Maddocks D, Jones M, Abdel Hadi M, Abdel-Fattah S, Avent N, Soothill P.W. Free fetal DNA in maternal plasma in anembryonic pregnancies: confirmation that the origin is the trophoblast. Prenat. Diagn. 2007;27(5):415-418.

    Article  CAS  Google Scholar 

  5. Chan KC, Ding C, Gerovassili A, Yeung SW, Chiu RW, Leung TN, Lau TK, Chim SS, Chung GT, Nicolaides KH, Lo YM. Hypermethylated RASSF1A in maternal plasma: A universal fetal DNA marker that improves the reliability of noninvasive prenatal diagnosis. Clin. Chem. 2006;52(12):2211-2218.

    Article  CAS  Google Scholar 

  6. Galbiati S, Smid M, Gambini D, Ferrari A, Restagno G, Viora E, Campogrande M, Bastonero S, Pagliano M, Calza S, Ferrari M, Cremonesi L. Fetal DNA detection in maternal plasma throughout gestation. Hum. Genet. 2005;117(2-3):243-248.

    Article  CAS  Google Scholar 

  7. Jakobsen TR, Clausen FB, Rode L, Dziegiel MH, Tabor A. Identifying mild and severe preeclampsia in asymptomatic pregnant women by levels of cell-free fetal DNA. Transfusion. 2013;53(9):1956-1964.

    Article  CAS  Google Scholar 

  8. Kim MJ, Kim SY, Park SY, Ahn HK, Chung JH, Ryu HM. Association of fetal-derived hypermethylated RASSF1A concentration in placenta-mediated pregnancy complications. Placenta. 2013;34(1):57-61.

    Article  CAS  Google Scholar 

  9. Lo YM, Corbetta N, Chamberlain PF, Rai V, Sargent IL, Redman CW, Wainscoat JS. Presence of fetal DNA in maternal plasma and serum. Lancet. 1997;350:485-487.

    Article  CAS  Google Scholar 

  10. Lo YM, Tein MS, Lau TK, Haines CJ, Leung TN, Poon PM, Wainscoat JS, Johnson PJ, Chang AM, Hjelm NM. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am. J. Hum. Genet. 1998;62(4):768-775.

    Article  CAS  Google Scholar 

  11. Mannaerts D, Faes E, Gielis J, Van Craenenbroeck E, Cos P, Spaanderman M, Gyselaers W, Cornette J, Jacquemyn Y. Oxidative stress and endothelial function in normal pregnancy versus pre-eclampsia, a combined longitudinal and case control study. BMC Pregnancy Childbirth. 2018;18(1):60. doi: https://doi.org/10.1186/s12884-018-1685-5.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Rolnik DL, O’Gorman N, Fiolna M, van den Boom D, Nicolaides KH, Poon LC. Maternal plasma cell-free DNA in the prediction of pre-eclampsia. Ultrasound Obstet. Gynecol. 2015;45(1):106-111.

    Article  CAS  Google Scholar 

  13. Smith S.C, Baker P.N, Symonds E.M. Placental apoptosis in normal human pregnancy. Am. J. Obstet. Gynecol. 1997; 177(1):57-65.

    Article  CAS  Google Scholar 

  14. Zhao F, Wang J, Liu R, Yang J, Cui K, Wu Y, Guo J, Mu Y, Wang X. Quantification and application of the placental epigenetic signature of the RASSF1A gene in maternal plasma. Prenat. Diagn. 2010;30(8):778-782.

    Article  Google Scholar 

  15. Zhou Y, Zhu Z, Gao Y, Yuan Y, Guo Y, Zhou L, Liao K, Wang J, Du B, Hou Y, Chen Z, Chen F, Zhang H, Yu C, Zhao L, Lau TK, Jiang F, Wang W. Effects of maternal and fetal characteristics on cell-free fetal DNA fraction in maternal plasma. Reprod. Sci. 2015;22(11):1429-1435.

    Article  Google Scholar 

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Authors and Affiliations

  1. V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    А. О. Karapetyan, О. R. Baev, А. М. Krasnyi, А. А. Sadekova & S. М. Мullabaeva

  2. I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation, Moscow, Russia

    О. R. Baev

  3. N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    А. М. Krasnyi

Authors
  1. А. О. Karapetyan
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  2. О. R. Baev
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  3. А. М. Krasnyi
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  4. А. А. Sadekova
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  5. S. М. Мullabaeva
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Correspondence to А. О. Karapetyan.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 7, pp. 103-106, July, 2017

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Karapetyan, А.О., Baev, О.R., Krasnyi, А.М. et al. Extracellular DNA in the Dynamics of Uncomplicated Pregnancy. Bull Exp Biol Med 166, 92–95 (2018). https://doi.org/10.1007/s10517-018-4295-3

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  • DOI: https://doi.org/10.1007/s10517-018-4295-3

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