Colorectal tumors were studied 5 months after carcinogen injection to outbred albino rats with secondary immunodeficiency by common histological and immunobiochemical methods with the use of monoclonal and polyclonal antibodies to Ki-67, Bcl-2, p53, and VEGF. Injection of carcinogen to intact animals led to the formation of adenocarcinomas with high and moderate cell differentiation in the colon, while injection of carcinogen to animals with immunodeficiency was associated with a 1.5-fold higher incidence of GRADE 2 and GRADE 3 adenocarcinomas with more aggressive morphological phenotype and appearance of distant metastases.
Similar content being viewed by others
References
Barsukov YuA, Tkachev SI, Oltarzhevskaya ND, Kuzmichev DV, Mamedli ZZ, Pavlova AM, Malikhov AG, Kholyavka EN. Neoadjuvant thermochemoradiotherapy using intrarectal 5-fluoruracil in combined treatment of rectal cancer. Onkol. Koloproktol. 2013;(1):9-17. Russian.
Gorban NA, Popuchiev VV, Baryshev VV. Prognostic criteria for the course of squamous cell carcinoma of the larynx (a review of literature). Opukholi Golovy Shei. 2013;(1):33-38. Russian.
Egorenkov VV. Prevention of stomach and colon cancer. Prakt. Onkol. 2011;12(2):70-75. Russian.
Zabezhimskii MA. Principles of primary cancer prevention. Prakt. Onkol. 2011;12(2):57-61. Russian.
Health Care in the Russian Federation. Statistical Compendium. Dianov VA, ed. Moscow, 2015. Russian.
Immunohistochemical Methods: Guideline. Kumar GL, Rudbeck L, eds. Moscow, 2011. Russian.
Kostrova OY, Mikhaylova MN, Struchko GY, Merkulova LM, Bessonova KV, Drandrova EG, Stomenskaya IS. Accidental thymic involution in rats in the background of a colon adenocarcinoma induced by 1,2-dimethylhydrazine development after removal of the spleen. Vestn. Chuvash. Univer. 2012;(3):416-423. Russian.
Mikhaylova MN, Struchko GY, Merkulova LM, Kostrova OY, Moskvichev EV, Drandrova EG, Stomenskaya IS. Involvment of dendritic and neuroendocrinic cells of thymus in the development of its involution against the formation of experimental colon cancer. Vestn. Chuvash. Univer. 2011;(3):377-383. Russian.
Moskvichev EV, Merkulova LM, Struchko GYu, Stomenskaya IS. Experimental carcinogenesis in condition of secondary immunodeficiency. Morfol. Vedomosti. 2009;(3-4):72-74.
Nevozhaj DV, Budzynskaya R, Kan’skaya U, Yagello M, Boratyn’sky Yu. Modern ideas about the mechanism of antineoplastic action of methotrexate and resistance to it. Tikhookean. Med. Zh. 2006;(4):12-16. Russian.
Struchko GYu, Merkulova LM, Moskvichyov YeV. Morphological and immunohistochemical characteristics of the thymus during chemical carcinogenesis induced by 1,2-dimethylhydrazine administration. Morfologiya. 2014;146(5):35-39. Russian.
Chesnokova N, Morrison VV, Barsukov VYu, Ponukalina EV, Agabekov AI. Modern concepts on carcinogenesis: the value of insufficiency of malignant cell elimination mechanisms in the tumor progression in case of neoplasia at different localizations. Saratov. Nauch.-Med. Zh. 2016;12(1):32-35. Russian.
Muller PA, Vousden KH. Mutant p53 in cancer: new functions and therapeutic opportunities. Cancer Cell. 2014;25(3):304-317.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 7, pp. 84-89, July, 2018
Rights and permissions
About this article
Cite this article
Struchko, G.Y., Merkulova, L.M., Moskvichev, E.V. et al. New Experimental Facts on the Influence of Secondary Immunodeficiency on the Morphology and Biological Activity of Colorectal Tumor. Bull Exp Biol Med 166, 75–79 (2018). https://doi.org/10.1007/s10517-018-4292-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4292-6