Skip to main content
SpringerLink
Log in

Combined Effect of TLR2 Ligands on ROS Production by Mouse Peritoneal Macrophages

  • BIOPHYSICS AND BIOCHEMISTRY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

TLR2-mediated ROS production by mouse peritoneal macrophages was studied by luminoldependent chemiluminescence under conditions of cell stimulation with zymosan (TLR2/6 ligand) and peptidoglycan (TLR2/1 ligand). ROS production by macrophages stimulated with zymosan and peptidoglycan simultaneously depended on the ratio of ligand concentrations. Three effects were revealed: additivity of the stimulating effects of the ligands used, competitive ligand binding, and effect of macrophage priming with peptidoglycan during cell stimulation with zymosan. The mechanisms of these effects are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Immunological Methods. Frimel G, ed. Moscow, 1987. Russian.

  2. Abdul-Cader MS, Amarasinghe A, Abdul-Careem MF. Activation of toll-like receptor signaling pathways leading to nitric oxide-mediated antiviral responses. Arch. Virol. 2016; 161(8):2075-2086.

    Article  CAS  Google Scholar 

  3. El-Benna J, Hurtado-Nedelec M, Marzaioli V, Marie JC, Gougerot-Pocidalo MA, Dang PM. Priming of the neutrophil respiratory burst: role in host defense and inflammation. Immunol. Rev. 2016;273(1):180-193.

    Article  CAS  Google Scholar 

  4. Hayashi F, Means TK, Luster AD. Toll-like receptors stimulate human neutrophil function. Blood. 2003;102(7):2660-2669.

    Article  CAS  Google Scholar 

  5. Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol. 2010;11(5):373-384.

    Article  CAS  Google Scholar 

  6. Kurt-Jones EA, Mandell L, Whitney C, Padgett A, Gosselin K, Newburger PE, Finberg RW. Role of toll-like receptor 2 (TLR2) in neutrophil activation: GM-CSF enhances TLR2 expression and TLR2-mediated interleukin 8 responses in neutrophils. Blood. 2002;100(5):1860-1868.

  7. Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J. Leukoc. Biol. 2004;75(3):467-477.

    Article  CAS  Google Scholar 

  8. Makni-Maalej K, Chiandotto M, Hurtado-Nedelec M, Bedouhene S, Gougerot-Pocidalo MA, Dang PM, El-Benna J. Zymosan induces NADPH oxidase activation in human neutrophils by inducing the phosphorylation of p47phox and the activation of Rac2: involvement of protein tyrosine kinases, PI3Kinase, PKC, ERK1/2 and p38MAPkinase. Biochem. Pharmacol. 2013;85(1):92-100.

    Article  CAS  Google Scholar 

  9. Martínez-Martínez L, Timmerman CP, Fleer A, Verhoef J. Chemiluminescence of human polymorphonuclear leucocytes after stimulation with whole cells and cell-wall components of Staphylococcus epidermidis. J. Med. Microbiol. 1993;39(3):196-203.

    Article  Google Scholar 

  10. Paradis A, Bernier S, Dumais N. TLR4 induces CCR7-dependent monocytes transmigration through the blood-brain barrier. J. Neuroimmunol. 2016;295-296:12-17.

    Article  CAS  Google Scholar 

  11. Remer KA, Reimer T, Brcic M, Jungi TW. Evidence for involvement of peptidoglycan in the triggering of an oxidative burst by Listeria monocytogenes in phagocytes. Clin. Exp. Immunol. 2005;140(1):73-80.

    Article  CAS  Google Scholar 

  12. Sheppard FR, Kelher MR, Moore EE, McLaughlin NJ, Banerjee A, Silliman CC. Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation. J. Leukoc. Biol. 2005;78(5):1025-1042.

    Article  CAS  Google Scholar 

  13. Smith SM. Role of Toll-like receptors in Helicobacter pylori infection and immunity. World J. Gastrointest. Pathophysiol. 2014;5(3):133-146.

    Article  Google Scholar 

  14. Vladimirov YA, Proskurnina EV. Free radicals and cell chemiluminescence. Biochemistry (Mosc). 2009;74(13):1545-1566.

    Article  CAS  Google Scholar 

  15. Xiang M, Fan J, Fan J. Association of Toll-like receptor signaling and reactive oxygen species: a potential therapeutic target for posttrauma acute lung injury. Mediators Inflamm. 2010;2010. pii: 916425. doi: https://doi.org/10.1155/2010/916425.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    Yu. O. Teselkin, M. V. Khoreva, A. V. Veselova, I. V. Babenkova, A. N. Osipov, L. V. Gankovskaya & Yu. A. Vladimirov

Authors
  1. Yu. O. Teselkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Khoreva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Veselova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. V. Babenkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. N. Osipov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. V. Gankovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yu. A. Vladimirov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu. O. Teselkin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 7, pp. 30-35, July, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Teselkin, Y.O., Khoreva, M.V., Veselova, A.V. et al. Combined Effect of TLR2 Ligands on ROS Production by Mouse Peritoneal Macrophages. Bull Exp Biol Med 166, 26–30 (2018). https://doi.org/10.1007/s10517-018-4281-9

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4281-9

Key Words

Search

Navigation