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Immunohistochemical Phenotypes of Stable and Unstable Occlusive Atherosclerotic Plaques in Coronary Arteries

Bulletin of Experimental Biology and Medicine volume 165, pages 798–802 (2018)Cite this article

We performed a complex morphological analysis of atherosclerotic plaques obtained from 68 men with coronary atherosclerosis during coronary bypass surgery with endarterectomy. The expression of MMP-2 and MMP-9, collagen IV, CD31, CD34, factor VIII, and of smooth muscle cell actin was measured in the samples by morphometric and immunohistochemical methods. The expression of MMP-2 and MMP-9 as well as the intensity of neoangiogenesis estimated by the expression of CD31, CD34, and factor VIII in unstable plaques was significantly higher than in stable ones. Immunohistochemical analysis showed more intensive collagen IV expression in stable plaques. The observed differences in immunohistochemical phenotypes of stable and unstable atherosclerotic plaques reflect peculiarities of morphogenesis of atherosclerotic foci in the coronary arteries determining their further development.

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Affiliations

  1. Institute of Molecular Pathology and Pathophysiology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia

    I. S. Murashov, S. V. Klochkova & E. L. Lushnikova

  2. E. N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia

    I. S. Murashov, A. M. Volkov, G. M. Kazanskaya & E. E. Kliver

  3. Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia

    S. V. Savchenko

Authors
  1. I. S. Murashov
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  2. A. M. Volkov
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  3. G. M. Kazanskaya
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  4. E. E. Kliver
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  5. S. V. Savchenko
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  6. S. V. Klochkova
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  7. E. L. Lushnikova
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Correspondence to I. S. Murashov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 6, pp. 762-767, June, 2018

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Murashov, I.S., Volkov, A.M., Kazanskaya, G.M. et al. Immunohistochemical Phenotypes of Stable and Unstable Occlusive Atherosclerotic Plaques in Coronary Arteries. Bull Exp Biol Med 165, 798–802 (2018). https://doi.org/10.1007/s10517-018-4268-6

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  • DOI: https://doi.org/10.1007/s10517-018-4268-6

Key Words

  • atherosclerotic plaques
  • matrix metalloproteinases
  • collagen IV
  • neoangiogenesis
  • immunohistochemistry