We studied viability of adenosine-modified monocytes after cryopreservation and their regenerative potential. The optimal method of cell cryopreservation was the use of serum-free medium followed by storage in liquid nitrogen. Injections of adenosine-modified monocytes after 12-week cryopreservation around grade IIIB burn wound accelerated healing of the skin defect and significantly improved the histological pattern of the damage area at delayed terms.
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Kutsenko SA, Daniliv MV, Basharin VA, Ivanov MB, Vasilevich SV, Zargarova NI. Experimental evaluation of the efficacy of Arnica preparations: cream Arniec, gel Arniec, liniment Arniec in mechanical, thermal and radiation injuries of the skin. Medline.ru. 2007;8(1):579-615. Russian.
Bhardwaj N, Chouhan D, Mandal BB. Tissue engineered skin and wound healing; current strategies and future directions. Curr. Pharm. Des. 2017;23(24):3455-3482.
Delirezh N, Shojaeefar E, Parvin P, Asadi B. Comparison the effects of two monocyte isolation methods, plastic adherence and magnetic activated cell sorting methods, on phagocytic activity of generated dendritic cells. Cell J. 2013;15(3):218-223.
Ghieh F, Jurjus R, Ibrahim A, Geagea AG, Daouk H, El Baba B, Chams S, Matar M, Zein W, Jurjus A. The use of stem cells in burn wound healing: A review. Biomed. Res. Int. 2015;2015. ID 684084. doi: https://doi.org/10.1155/2015/684084.
Haskó G, Pacher P. Regulation of macrophage function by adenosine. Arterioscler. Thromb. Vasc. Biol. 2012;32(4):865-869.
Ogle ME, Segar CE, Sridhar S, Botchwey EA. Monocytes and macrophages in tissue repair: Implications for immunoregeneraive biomaterial design. Exp. Biol. Med. (Maywood). 2016;241(10):1084-1097.
Ozturk S, Karagoz H. Experimental stem cell therapies on burn wound: do source, dose, timing and method matter. Burns. 2015;41(6):1133-1139.
Peck MD. Epidemiology of burns throughout the world. Part I: distribution and risk factors. Burns. 2011;37(7):1087-1100.
Smolle C, Cambiaso-Daniel J, Forbes AA, Wurzer P, Hundeshagen G, Branski LK, Huss F, Kamolz LP. Recent trends in burn epidemiology worldwide: A systematic review. Burns. 2017;43(2):249-257.
Trounson A, McDonald C. Stem cell therapies in clinical trials: progress and challenges. Cell Stem Cell. 2015;17(1):11-22.
Vig K, Chaudhari A, Tripathi S, Dixit S, Sahu R, Pillai S, Dennis VA, Singh SR. Advances in skin regeneration using tissue engineering. Int. J. Mol. Sci. 2017;18(4):pii: E789. doi: https://doi.org/10.3390/ijms18040789.
Yuryeva KS, Nevskaya KV, Dzuman AN, Ikkert OP, Ivanov VV, Saltikova IV, Sazonov AE, Ogorodova LM. Stimulation of adenosine receptors on myeloid cells enhances leukocyte migration at the site of burn injury. Biochemistry (Mosc). Suppl. Ser. B: Biomed. Chem. 2014;8(4):336-342.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 4, pp. 524-528, April, 2018
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Nevskaya, K.V., Ivanov, V.V., Mainagasheva, E.S. et al. Evaluation of Regenerative Potential of Cryopreserved Adenosine-Modified Monocytes on the Model of Burn Wounds. Bull Exp Biol Med 165, 526–529 (2018). https://doi.org/10.1007/s10517-018-4209-4
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DOI: https://doi.org/10.1007/s10517-018-4209-4