Pilot experiments on kinetic vitrification of human pluripotent stem cells and spermatozoa using a KrioBlastTM-2 without penetrating cryoprotectants have shown high survival of cells (75-85% in both cases).
Similar content being viewed by others
Change history
23 June 2018
The title of the article should be «KrioBlastTM as a New Technology of Hyper-fast Cryopreservation of Cells and Tissues. Part II. 2. Kinetic Vitrification of Human Pluripotent Stem Cells and Spermatozoa».
References
Katkov II, Bolyukh VF, Sukhikh GT. KrioBlast TM as a New Technology of Hyper-Fast Cryopreservation of Cells and Tissues. Part I. Thermodynamic Aspects and Potential Applications in Reproductive and Regenerative Medicine. Bull. Exp. Biol. Med. 2018;164(4):530-535.
Katkov II, Bolyukh VF. Patent RU No. 2624963. Device for super-quick cooling of biological samples up to cryogenic temperatures. Bull. No. 20. Published July 11, 2017.
Isachenko E, Isachenko V, Katkov II. Vitrification in Assisted Reproduction: a User’s Manual and Troubleshooting Guide. Tucker MJ, Liebermann J, eds. Oxon, 2007. P. 87-105.
Isachenko E, Isachenko V, Katkov I.I, Dessole S, Nawroth F. Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reprod. Biomed. Online. 2003;6(2):191-200.
Isachenko E, Isachenko V, Sanchez R, Katkov II, Kreienberg, R. Cryopreservation of spermatozoa: old routine and new perspective. Principles and practice of fertility preservation. Donnez J, Kim SS, eds. Cambridge, 2011. P. 177-199.
Katkov II. Race for the pace: is the universal cryoprotocol a dream or reality? Cryobiology 2010;61:374-375.
Katkov II, Bolyukh VF. Method and scalable devices for hyperfast cooling and warming. US Patent 9,638,452 B2, 2017, Assignee: CELLTRONIX, priority September 9, 2012.
Katkov II, Bolyukh AF, Chernetsov OA, Dudin PI, Grigoriev AY, Isachenko V, Isachenko E, Lulat AGM, Moskovtsev SI, Petrushko MP, Pinyaev VI, Sushko AB, Sokol KM, Sokol YI, Yakhnenko I. Kinetic vitrification of spermatozoa of vertebrates: what can we learn from nature? Current frontiers in cryobiology. Katkov II, ed. InTech Open Access Books, 2012;(Ch. 1):3-40. URL: http://www.intechopen.com/books/current-frontiers-in-cryobiology.
Warkentin M, Stanislavskaia V, Hammes K, Thorne RE. Crystallography in capillaries: critical glycerol concentrations and cooling rates. J. Appl. Cryst. 2008;41:791-797.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 63-68, January, 2018
Rights and permissions
About this article
Cite this article
Katkov, I.I., Bolyukh, V.F. & Sukhikh, G.T. KrioBlast TM as a New Technology of Ultrafast Cryopreservation of Cells and Tissues. 2. Kinetic Vitrification of Human Pluripotent Stem Cells and Spermatozoa. Bull Exp Biol Med 165, 171–175 (2018). https://doi.org/10.1007/s10517-018-4122-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4122-x