Skip to main content
Log in

KrioBlast TM as a New Technology of Ultrafast Cryopreservation of Cells and Tissues. 2. Kinetic Vitrification of Human Pluripotent Stem Cells and Spermatozoa

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

A Correction to this article was published on 23 June 2018

This article has been updated

Pilot experiments on kinetic vitrification of human pluripotent stem cells and spermatozoa using a KrioBlastTM-2 without penetrating cryoprotectants have shown high survival of cells (75-85% in both cases).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Change history

  • 23 June 2018

    The title of the article should be «KrioBlastTM as a New Technology of Hyper-fast Cryopreservation of Cells and Tissues. Part II. 2. Kinetic Vitrification of Human Pluripotent Stem Cells and Spermatozoa».

References

  1. Katkov II, Bolyukh VF, Sukhikh GT. KrioBlast TM as a New Technology of Hyper-Fast Cryopreservation of Cells and Tissues. Part I. Thermodynamic Aspects and Potential Applications in Reproductive and Regenerative Medicine. Bull. Exp. Biol. Med. 2018;164(4):530-535.

    Article  PubMed  CAS  Google Scholar 

  2. Katkov II, Bolyukh VF. Patent RU No. 2624963. Device for super-quick cooling of biological samples up to cryogenic temperatures. Bull. No. 20. Published July 11, 2017.

  3. Isachenko E, Isachenko V, Katkov II. Vitrification in Assisted Reproduction: a User’s Manual and Troubleshooting Guide. Tucker MJ, Liebermann J, eds. Oxon, 2007. P. 87-105.

  4. Isachenko E, Isachenko V, Katkov I.I, Dessole S, Nawroth F. Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reprod. Biomed. Online. 2003;6(2):191-200.

    Article  PubMed  Google Scholar 

  5. Isachenko E, Isachenko V, Sanchez R, Katkov II, Kreienberg, R. Cryopreservation of spermatozoa: old routine and new perspective. Principles and practice of fertility preservation. Donnez J, Kim SS, eds. Cambridge, 2011. P. 177-199.

  6. Katkov II. Race for the pace: is the universal cryoprotocol a dream or reality? Cryobiology 2010;61:374-375.

    Article  Google Scholar 

  7. Katkov II, Bolyukh VF. Method and scalable devices for hyperfast cooling and warming. US Patent 9,638,452 B2, 2017, Assignee: CELLTRONIX, priority September 9, 2012.

  8. Katkov II, Bolyukh AF, Chernetsov OA, Dudin PI, Grigoriev AY, Isachenko V, Isachenko E, Lulat AGM, Moskovtsev SI, Petrushko MP, Pinyaev VI, Sushko AB, Sokol KM, Sokol YI, Yakhnenko I. Kinetic vitrification of spermatozoa of vertebrates: what can we learn from nature? Current frontiers in cryobiology. Katkov II, ed. InTech Open Access Books, 2012;(Ch. 1):3-40. URL: http://www.intechopen.com/books/current-frontiers-in-cryobiology.

  9. Warkentin M, Stanislavskaia V, Hammes K, Thorne RE. Crystallography in capillaries: critical glycerol concentrations and cooling rates. J. Appl. Cryst. 2008;41:791-797.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to I. I. Katkov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 63-68, January, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Katkov, I.I., Bolyukh, V.F. & Sukhikh, G.T. KrioBlast TM as a New Technology of Ultrafast Cryopreservation of Cells and Tissues. 2. Kinetic Vitrification of Human Pluripotent Stem Cells and Spermatozoa. Bull Exp Biol Med 165, 171–175 (2018). https://doi.org/10.1007/s10517-018-4122-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4122-x

Key Words

Navigation