We studied the effect of endothelial cells on in vitro migration and differentiation potential of multipotent mesenchymal stromal cells. Down-regulation of stemness genes OCT4, SOX2, and chondrogenic differentiation regulator SOX9 gene and upregulation of osteogenesis master-gene RUNX2 in mesenchymal stromal cells were observed in the presence of intact and TNFα-activated endothelial cells, which indicated an increase in commitment of mesenchymal stromal cells.The medium conditioned by endothelial cells stimulated migration activity of mesenchymal stromal cells; migration rate increased significantly in conditioned medium from activated cells in comparison with medium from non-activated cells. It was concluded that the interaction with endothelial cells modulated functional activity of mesenchymal stromal cells; moreover, activated endothelial cells produced more pronounced effects on differentiation potential and migration activity of mesenchymal stromal cells both in direct contact and through paracrine regulation.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 15-19, January, 2018
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Zhidkova, O.V., Andreeva, E.R. & Buravkova, L.B. Endothelial Cells Modulate Differentiation Potential and Mobility of Mesenchymal Stromal Cells. Bull Exp Biol Med 165, 127–131 (2018). https://doi.org/10.1007/s10517-018-4113-y
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DOI: https://doi.org/10.1007/s10517-018-4113-y