In Vivo Experimental Study of Biological Compatibility of Tissue Engineered Tracheal Construct in Laboratory Primates
Biological compatibility of a tissue engineered construct of the trachea (synthetic scaffold) and allogenic mesenchymal stem cells was studied on laboratory Papio hamadryas primates. Subcutaneous implantation and orthotopic transplantations of tissue engineered constructs were carried out. Histological studies of the construct showed chaotically located filaments and mononuclear cells fixed to them. Development of a fine connective tissue capsule was found at the site of subcutaneous implantation of the tissue engineered construct. The intact structure of the scaffold populated by various cell types in orthotopic specimens was confirmed by expression of specific proteins. The results indicated biological compatibility of the tissue engineered construct with the mesenchymal stem cells; no tissue rejection reactions were recorded; simulation of respiratory disease therapy on Papio hamadryas proved to be an adequate model.
Key Wordstissue engineered tracheal construct primates mesenchymal stem cells subcutaneous test transplantation
Unable to display preview. Download preview PDF.
- 2.Baranovsky DS, Lyundup AV, Parshin VD. In vitro cultivation of functioning passaged ciliated epithelium for trachea tissue engineered. Веstn. Ross. Akad. Med. Nauk. 2015; 70(5):561-567. Russian.Google Scholar
- 3.Buharova TB, Volkov AV, Antonov EN, Vihrova EB, Popova AV, Popov VK, Goldstein DV. Tissue-engineered construction made of adipose derived multipotent mesenchymal stromal cells, polylactide scaffolds and platelet gel. Geny Kletki. 2013;8(4):61-68. Russian.Google Scholar
- 5.Gilevich IV, Porhanov VA. The properties of tissue-engineered trachea, composed from synthetic scaffold seeded with human autologous bone marrow mononuclear cells. Vestn. Ural. Med. Akad. Nauki. 2015;55(4):106-108. Russian.Google Scholar
- 7.Kiselevskii MV, Anisimova NYu, Kornyushenkov EA, Shepelev AD, Chvalun SN, Polotskii BE, Davydov MI. Biocompatible synthetic tracheal matrixes based on polymer ultrafiber materials colonized by mesenchymal multipotent cells. Sovremen. Tekhnol. Med. 2016;8(1):6-13. Russian.Google Scholar
- 8.Sergeeva NS, Komlev VS, Sviridova IK, Kirsanova VA, Akhmedova SA, Shanskiy YaD, Kuvshinova EA, Fedotov AYu, Teterina AYu, Egorov AA, Zobkov YuV, Barinov SM. Some physicochemical and biological characteristics of 3D printed constructions based on sodium alginate and calcium phosphates for bone defects reconstruction. Geny Kletki. 2015;10(2):39-45.Google Scholar
- 10.Ajalloueian F, Lim ML, Lemon G, Haag JC, Gustafsson Y, Sjöqvist S, Beltrán-Rodríguez A, Del Gaudio C, Baiguera S, Bianco A, Jungebluth P, Macchiarini P. Biomechanical and biocompatibility characteristics of electrospun polymeric tracheal scaffolds. Biomaterials. 2014;35(20):5307-5315.CrossRefPubMedGoogle Scholar
- 13.Gustafsson Y, Haag J, Jungebluth P, Lundin V, Lim ML, Baiguera S, Ajalloueian F, Del Gaudio C, Bianco A, Moll G, Sjöqvist S, Lemon G, Teixeira AI, Macchiarini P. Viability and proliferation of rat MSCs on adhesion protein-modified PET and PU scaffolds. Biomaterials. 2012;33(32):8094-8103.CrossRefPubMedGoogle Scholar