Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 6, pp 716–720 | Cite as

Effect of Natural Cytokine Complex on the Structure and Metabolism of the Cardiac Conduction System in the Myocardium under Normally and Increased Hemodynamic Load

  • M. S. Tverskaya
  • L. V. Gankovskaya
  • V. V. Sukhoparova
  • A. O. Virganskii
Article
  • 20 Downloads

Effect of natural complex of cytokines with activity of IL-1, IL-2, IL-6, TNF, MIF, and GTFβ on the structure and metabolism of conduction cardiomyocytes was assessed in the control and under acute experimental aortic stenosis. After systemic administration of the cytokine complex in the control, structural abnormalities were revealed in a relatively low number of conduction cardiomyocytes; their relative number increased in the left ventricle and interventricular septum. When the complex was administered against the background of aortic stenosis, morphological changes in the conduction system were seen in a significant number of cells with their plasma imbibition, especially in the left ventricle and interventricular septum. Systemic administration of the natural cytokine complex inhibited the major metabolic processes in the conduction system, both in the control and under conditions of sharply increased hemodynamic load. In conduction cardiomyocytes, deceleration of glycolysis and citric acid cycle, inhibition of oxidation of free fatty acids and their metabolites, and suppression of shuttle mechanisms and biosynthetic reactions were observed. Increased blood levels of cytokines, primarily of the proinflammatory ones, can cause structural and metabolic disturbances in the cardiac conduction system and promote the development of arrhythmias, especially in case of sharply increased hemodynamic load.

Key Words

cardiac conduction system structure metabolism cytokines 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anderson RH, Ho SY, Wharton J, Becker AE. Gross anatomy and microscopy of the conduction system. Cardiac Arrhythmias. Vol. 1. Moscow, 1996. P. 40-106. Russian.Google Scholar
  2. 2.
    Koval’chuk LV, Gankovskaya LV, Meshkova RYa. Fundamentals of immunotherapy. Clinical Immunology and Allergology with the Basics of General Immunology. Moscow, 2011. P. 588-615. Russian.Google Scholar
  3. 3.
    Nikankina LV, Dolgina EN, Gankovskaia LV, Klebanov GI, Koval’chuk LV. The modulation of phagocyte oxygen metabolism by recombinant cytokines and a complex of natural cytokines. Zh. Mikrobiol. Epidemiol. Immunobiol. 1999;(5):106-108. Russian.Google Scholar
  4. 4.
    Simbirtsev AS. Oytokines in the pathogenesis of infectious and noninfectious human diseases. Med. Akad. Zh. 2013;13(3):18-41. Russian.Google Scholar
  5. 5.
    Tverskaya MS, Gankovskaya LV, Sukhoparova VV, Virganskii AO. Effect of the Natural Cytokine Complex on the Structure and Metabolism of Contractile Myocardium Normally and under Increased Hemodynamic Load. Bull. Exp. Biol. Med. 2017;164(2):136-139.CrossRefPubMedGoogle Scholar
  6. 6.
    Tverskaya MS, Sukhoparova VV, Kadyrova MKh, Klyuchikov VY, Bobrova NA. Histoenzymological characteristics of the heart conduction system: comparative study with left or right ventricle afterload. Bull. Exp. Biol. Med. 2013;155(5):618-621.CrossRefPubMedGoogle Scholar
  7. 7.
    Tverskaya MS, Sukhoparova VV, Karpova VV, Kadyrova MKh, Klyuchikov VY. Pathomorphology of the heart conduction system: comparative study during increase in left or right ventricular afterload. Bull. Exp. Biol. Med. 2011;151(5):634-637.CrossRefPubMedGoogle Scholar
  8. 8.
    Tverskaya MS, Sukhoparova VV, Karpova VV, Raksha AP, Kadyrova MK, Abdulkerimova NZ, Bobrova NA. Pathomorphology of myocardial circulation: comparative study in increased left or right ventricle afterload. Bull. Exp. Biol. Med. 2008;145(3):377-381.CrossRefPubMedGoogle Scholar
  9. 9.
    Aviles RJ, Martin DO, Apperson-Hansen C, Houghtaling PL, Rautaharju P, Kronmal RA, Tracy RP, Van Wagoner DR, Psaty BM, Lauer MS, Chung MK. Inflammation as a risk factor for atrial fibrillation. Circulation. 2003;108(24):3006-3010.CrossRefPubMedGoogle Scholar
  10. 10.
    Duncan DJ, Yang Z, Hopkins PM, Steele DS, Harrison SM. TNF-alpha and IL-1beta increase Ca2+ leak from the sarcoplasmic reticulum and susceptibility to arrhythmia in rat ventricular myocytes. Cell Calcium. 2010;47(4):378-386.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Kuzmin VS, Abramochkin DV, Mitrochin VM, Tian B, Makarenko EYu, Kovalchuk LV, Khoreva MV, Nikonova A, KaluginL, Lysenko NN, Lozinsky I, Rozanov A, Arutyunov G, Kiseleva I, Kamkin A. The role of proinflammatory cytokines in regulation of cardiac bioelectrical activity: link to mechanoelectrical feedback. Mechanical Stretch and Cytokines. Mechanosensitivity in Cells and Tissues. Vol. 5. Dordrecht, 2012. P. 107-154.Google Scholar
  12. 12.
    Lee SH, Chen YC, Chen YJ, Chang SL, Tai CT, Wongcharoen W, Yeh HI, Lin CI, Chen SA. Tumor necrosis factor-alpha alters calcium handling and increases arrhythmogenesis of pulmonary vein cardiomyocytes. Life Sci. 2007;80(19):1806-1815.CrossRefPubMedGoogle Scholar
  13. 13.
    Marcus GM, Whooley MA, Glidden DV, Pawlikowska L, Zaroff JG, Olgin JE. Interleukin-6 and atrial fibrillation in patients with coronary artery disease: data from the Heart and Soul Study. Am. Heart J. 2008;155(2):303-309.CrossRefPubMedGoogle Scholar
  14. 14.
    Saba S, Janczewski AM, Baker LC, Shusterman V, Gursoy EC, Feldman AM, Salama G, McTiernan CF, London B. Atrial contractile dysfunction, fibrosis and arrhythmias in a mouse model of cardiomyopathy secondary to cardiac-specific over-expression of tumor necrosis factor-α. Am. J. Physiol. Heart Circ. Physiol. 2004;289(4):H1456-H1467.CrossRefGoogle Scholar
  15. 15.
    Yu X, Patterson E, Huang S, Garrett MW, Kem DC. Tumor necrosis factor-α, rapid ventricular tachyarrhythmias and infarct size in canine models of myocardial infarction. J. Cardiovasc. Pharmacol. 2005;45(2):153-159.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • M. S. Tverskaya
    • 1
  • L. V. Gankovskaya
    • 1
  • V. V. Sukhoparova
    • 1
  • A. O. Virganskii
    • 1
  1. 1.N. I. Pirogov Russian National Research Medical UniversityMoscowRussia

Personalised recommendations