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KrioBlast TM as a New Technology of Hyper-fast Cryopreservation of Cells and Tissues. Part I. Thermodynamic Aspects and Potential Applications in Reproductive and Regenerative Medicine

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Kinetic (dynamic) vitrification is a promising trend in cryopreservation of biological materials because it allows avoiding the formation of lethal intracellular ice and minimizes harmful effects of highly toxic penetrating cryoprotectants. A uniform cooling protocol and the same instruments can be used for practically all types of cells. In modern technologies, the rate of cooling is essentially limited by the Leidenfrost effect. We describe a novel platform for kinetic vitrification of biological materials KrioBlastTM that realizes hyper-fast cooling and allows overcoming the Leidenfrost effect. This opens prospects for creation of a novel technology of cell cryopreservation for reproductive and regenerative medicine.

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Authors and Affiliations

  1. Belgorod State National Research University, Belgorod, Russia

    I. I. Katkov

  2. CELLTRONIX, San Diego, California, USA

    I. I. Katkov & V. F. Bolyukh

  3. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    G. T. Sukhikh

Authors
  1. I. I. Katkov
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  2. V. F. Bolyukh
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  3. G. T. Sukhikh
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Correspondence to I. I. Katkov.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 216-221, October, 2017

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Katkov, I.I., Bolyukh, V.F. & Sukhikh, G.T. KrioBlast TM as a New Technology of Hyper-fast Cryopreservation of Cells and Tissues. Part I. Thermodynamic Aspects and Potential Applications in Reproductive and Regenerative Medicine. Bull Exp Biol Med 164, 530–535 (2018). https://doi.org/10.1007/s10517-018-4027-8

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  • DOI: https://doi.org/10.1007/s10517-018-4027-8

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