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Peculiarities of Cell Seeding on Polylactic Acid-Based Scaffolds Fabricated Using Electrospinning and Solution Blow Spinning Technologies

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the possibility of seeding bone marrow-derived stromal cells onto polylactic acid-based scaffolds fabricated by electrospinning and solution blow spinning technologies. The cells were applied to the scaffolds by dynamic seeding and scaffolds were then cultured in Petri dishes in culture medium for 3 days. Cell migration to the Petri dish surface was noted only for scaffolds fabricated by electrospinning technology, but DAPI staining confirmed the presence of cells in both scaffolds. The mean number of cells in scaffolds fabricated by electrospinning and solution blow spinning was 56±9 and 81±6, respectively. The scaffold fabricated by solution blow spinning was more effectively (p<0.05) colonized by cells due to its more optimal spatial structure.

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Authors and Affiliations

  1. Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

    S. A. Afanasiev, E. F. Muslimova & Yu. V. Rogovskaya

  2. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    Yu. A. Nashchekina & P. O. Nikonov

  3. National Research Tomsk Polytechnical University, Tomsk, Russia

    E. N. Bolbasov & S. I. Tverdokhlebov

Authors
  1. S. A. Afanasiev
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  2. E. F. Muslimova
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  3. Yu. A. Nashchekina
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  4. P. O. Nikonov
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  5. Yu. V. Rogovskaya
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  6. E. N. Bolbasov
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  7. S. I. Tverdokhlebov
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Corresponding author

Correspondence to S. A. Afanasiev.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 192-195, July, 2017

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Afanasiev, S.A., Muslimova, E.F., Nashchekina, Y.A. et al. Peculiarities of Cell Seeding on Polylactic Acid-Based Scaffolds Fabricated Using Electrospinning and Solution Blow Spinning Technologies. Bull Exp Biol Med 164, 281–284 (2017). https://doi.org/10.1007/s10517-017-3973-x

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  • DOI: https://doi.org/10.1007/s10517-017-3973-x

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