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Characteristics of Trophoblasts in Long-Term Culture

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We analyzed more than 40 cytotrophoblast cultures derived from cell islets that grew from trypsinized tissue fragments of placental microvilli. Phenotypic variability of trophoblasts was demonstrated. Changes in trophoblast morphology from epithelium-like or oval cells to bipolar and spindle-shaped or twisted and then to mesenchymal-like cells as well as intensive expression of cytokeratin-7 and vimentin attested to epithelial-mesenchymal transition of trophoblasts during in vitro culturing. Analysis of the expression of specific markers in long-term trophoblast culture (≥7 passages) revealed the possibility of culture contamination with other non-trophoblast cells including fibroblasts. High risk of trophoblast culture contamination with rapidly growing cells necessitates regular control of the cultures used in fundamental studies. Our experiments confirmed the possibility of long-term culturing of cells maintaining trophoblast properties. The identity and purity of 4 trophoblast cultures free from contamination and retaining the properties of pure culture during long-term (>10 passages) culturing in vitro were confirmed.

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, Moscow, Russia

    T. D. Kolokol’tsova, I. N. Saburina, I. M. Zurina, A. A. Gorkun & N. V. Kosheleva

  2. University of Texas Medical Branch, Galveston, Texas, USA

    T. D. Kolokol’tsova, T. N. Nanovskaya, S. L. Patrikeeva & D. I. Vernikovskaya

  3. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    R. A. Poltavtseva & G. T. Sukhikh

  4. Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation, Moscow, Russia

    I. N. Saburina

Authors
  1. T. D. Kolokol’tsova
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  2. I. N. Saburina
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  3. T. N. Nanovskaya
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  4. S. L. Patrikeeva
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  5. D. I. Vernikovskaya
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  6. I. M. Zurina
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  7. A. A. Gorkun
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  8. N. V. Kosheleva
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  9. R. A. Poltavtseva
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  10. G. T. Sukhikh
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Corresponding author

Correspondence to T. D. Kolokol’tsova.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 168-174, July, 2017

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Kolokol’tsova, T.D., Saburina, I.N., Nanovskaya, T.N. et al. Characteristics of Trophoblasts in Long-Term Culture. Bull Exp Biol Med 164, 259–265 (2017). https://doi.org/10.1007/s10517-017-3969-6

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  • DOI: https://doi.org/10.1007/s10517-017-3969-6

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